Revista Mexicana de Ingeniería Química, Vol. 20, No. 3 (2021), Bio2407
α-L-Fucosidase from Thermotoga maritima: hydrolytic and transfucosylation activities
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M.A. Robles-Arias, F. Guzmán-Rodríguez, M. García-Garibay, S. Alatorre-Santamaría, L. Gómez-Ruiz, G. Rodríguez-Serrano, A.E. Cruz-Guerrero
https://doi.org/10.24275/rmiq/Bio2407
Abstract
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Fucosylated oligosaccharides play several biologically relevant roles. They are naturally present in human milk which offers to infants short- and long-term health benefits. These compounds can be obtained also by enzymatic synthesis. In this work, the effects of pH and temperature on hydrolytic and transfucosylation activities of α-L-fucosidase from Thermotoga maritima were evaluated. The optimal pH for the enzyme-catalyzed hydrolysis was found in a range from 6 to 8 while the highest conversions for transfucosylation reactions were observed within the range of 7-10. The best temperature for both enzymatic activities was 95 °C. Fucosylated oligosaccharides were synthesized with the highest productivity of 3.54 mM/h at pH 8 and 95 °C. Overall, optimization of the conditions of transfucosylation reaction catalyzed by the α-L-fucosidase from Thermotoga maritima allowed for higher yields of fucosylated oligosaccharides as well as shorter reaction time and a lower concentration of the employed enzyme.
Keywords: Fucosylated oligosaccharides, fucosidase, Thermotoga maritima.
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