Oxygen limitations to grow Azadirachta indica cell culture in shake flasks

 

F. Orozco-Sánchez, G. Sepúlveda-Jiménez, G. Trejo-Tapia, A. Zamilpa and M. Rodríguez-Monroy

 

 

  • Covers used for Erlenmeyer shake flasks (aluminium foil, cotton and silicone foam ) determine the oxygen transfer rate.

  • Cell growth of Azadirachta indica and their azadirachtins production are affected by the type of covers used in Erlenmeyer flasks.