It was evaluated the growth of Azadirachta cell suspension in different conditions of oxygen delivery in Erlenmeyer shake flask. Oxygen transfer rate (OTR, kg O₂ m^-3 day^-1) for the closures utilized were: silicone foam (1.04), cotton (0.58), and aluminum foil (0.07). A. indica ells growing during 6 weeks of subculture showed that lower OTR reduced cell viability, the pH of broth medium, and Azadirachtins production. While, higher OTR induced the formation of aggregates. Using a stirred tank bioreactor, it was determined that A. indica cells had an oxygen consumption of 0.100 kg O₂ kg CS^-1 day^-1, a higher value than other plant cell cultures. These results show that OTR generated in Erlenmeyer shake flasks is lower to oxygen uptake rate of A. indica cells and it is a limiting factor to grow this plant.