The interfacial shear viscosity (η^int) and the creep compliance-time (J(t)) behavior of milk fat globule membrane (MFGM) films (4, 5 and 6% w/w) formed at the water-oil interface were evaluated.\ Films with higher MFGM concentration displayed higher η^int and interfacial viscoelastic properties. When esters of polyglycerol and polyriciniolate fatty acids (PGPR) were added to the oil phase, a competitive adsorption at the interface took place between PGPR and MFGM which caused a decrease in the interfacial viscoelastic properties of the films. The change in the rheological behavior of the films suggests that their interfacial structure was determined by complex interactions between the MFGM and PGPR molecules. Water-in-oil-in-water multiple emulsions (ME) with smaller surface-volume droplet size (d_3,2), greater stability, and higher storage (G') and loss (G'') moduli were obtained when higher MFGM concentrations were used in the outer aqueous phase.