LACTOSE-INDUCED EXPRESSION OF RECOMBINANT TURNIP PEROXIDASE IN Escherichia coli
R. Pliego-Arreaga, C. Regalado, A. Amaro-Reyes, B.E. García-Almendárez
This is the first report using lactose as inducer of recombinant peroxidase which was purified by IMAC in a single step.
Peroxidase isoform (PodC) was heterologously expressed using Escherichia coli Rosetta 2 in a fermenter.
PodC from inclusion bodies was refolded under oxidative conditions and purified with a yield of 36 mg L-1 of culture.
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