LACTOSE-INDUCED EXPRESSION OF RECOMBINANT TURNIP PEROXIDASE IN Escherichia coli

 

R. Pliego-Arreaga, C. Regalado, A. Amaro-Reyes, B.E. García-Almendárez

 

 

  • This is the first report using lactose as inducer of recombinant peroxidase which was purified by IMAC in a single step.

  • Peroxidase isoform (PodC) was heterologously expressed using Escherichia coli Rosetta 2 in a fermenter.

  • PodC from inclusion bodies was refolded under oxidative conditions and purified with a yield of 36 mg L-1 of culture.