REFOLDING OF LYSOZYME ASSISTED BY MOLECULAR CHAPERONES IMMOBILIZED IN CELLULOSE: THE OPERATIONAL CONDITIONS THAT AFFECT REFOLDING YIELDS
A. Antonio-Pérez, L.M. Aldaz-Martínez, A. Meneses-Acosta and J. Ortega-López
Refolding of lysozyme assisted by the Apical Domain of GroEL, DsbA and DsbC immobilized in cellulose was investigated.
The effect of operational conditions on lysozyme refolding yield and stability of immobilized chaperones are presented.
Apical Domain of GroEL, DsbA and DsbC immobilized on cellulose assisted efficiently the refolding of lysozyme.
Chaperones immobilized on cellulose exhibited great stability at high concentrations of GdnHCl and β-ME.
Chaperones immobilized on cellulose are a cost-efficient alternative to refold proteins expressed as inclusion bodies.
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