REFOLDING OF LYSOZYME ASSISTED BY MOLECULAR CHAPERONES IMMOBILIZED IN CELLULOSE: THE OPERATIONAL CONDITIONS THAT AFFECT REFOLDING YIELDS

 

A. Antonio-Pérez, L.M. Aldaz-Martínez, A. Meneses-Acosta and J. Ortega-López

 

 

  • Refolding of lysozyme assisted by the Apical Domain of GroEL, DsbA and DsbC immobilized in cellulose was investigated.

  • The effect of operational conditions on lysozyme refolding yield and stability of immobilized chaperones are presented.

  • Apical Domain of GroEL, DsbA and DsbC immobilized on cellulose assisted efficiently the refolding of lysozyme.

  • Chaperones immobilized on cellulose exhibited great stability at high concentrations of GdnHCl and β-ME.

  • Chaperones immobilized on cellulose are a cost-efficient alternative to refold proteins expressed as inclusion bodies.