PRODUCTION AND OPTIMIZATION OF A CHLOROPHYL-FREE LEAF PROTEIN CONCENTRATE FROM ALFALFA (Medicago sativa) THROUGH AQUEOUS TWO-PHASE SYSTEM
P. Vázquez-Villegas, E. Acuña-González, L.A. Mejía-Manzano, M. Rito-Palomares, O. Aguilar
A chlorophyl-free leaf protein concentrate and powder was obtained from alfalfa.
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An aqueous two-phase systems (ATPS) process optimization was employed.
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To select the factors that minimize the partition coefficient, a two level factorial design experiment was performed.
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Steepest descent and central composite design (CCD) were used to optimize the ATPS.
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More than 80% and 51% of total protein were obtained for extract and powder, respectively.
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