The objective of this study was to separate the cellulose contained in the fique spall using different ligninolytic fungi fermented broth. The species Pleurotus sp. CBT1, Pleurotus sp. CBT2 and Pycnoporus sanguineous were adapted to a culture medium containing lignin as a carbon source.  The degradation of lignin in the culture medium was present between 12 and 72 h of fermentation for the three species, yielding degradation of 72, 60 and 54% for Pleurotus sp. CBT1, Pleurotus sp. CBT2 and Pycnoporus sanguineous respectively, which coincided with the maximum levels of enzyme activity on Mn-P of 4.59, 2.24 and 4.19 IU/L for Pleurotus sp. CBT1, Pleurotus sp. CBT2 and P. sanguineous, respectively. Later, fique spall was subjected to degradation in fermented broth Pleurotus sp. CBT1, Pleurotus sp. CBT2 and P. sanguineous at pH 5.0, temperature of 50 °C and 30 min of enzymatic action. The fermented broth from Pleurotus sp. CBT1 after 48 h of fermentation showed lignin degradation in the fique spall of 65.2 % higher than other species, decreasing the initial lignin content of 14.1 % to 4.9 % and an increase in cellulose content of 61.8 to 73.1%.