This is the first report about the production of lignocellulolytic enzymes of Pleurotus ostreatus grown in SSF using PUF as inert support.

Laccases were shown as constitutive enzymes, although the activity changed depending on the carbon sources used.

Cellulases were inducible and susceptible to catabolite repression, because they were not presented in the culture media with glucose only.

Glucose is important for growth of the fungus and xylan as inducer of xylanases.

Laccases are produced mainly in the stationary phase, cellulases in the exponential phase and xylanases at the beginning of growth.