Activities of laccase, cellulase and xylanase produced by Pleurotus ostreatus grown in solid-state fermentation using polyurethane foam as inert support were evaluated, and the growth kinetic parameters of fungus were obtained. In general, all enzymatic activities were observed in the fermentation broth. Laccases showed the highest activity (18,030 U L-1) in the medium with glucose as unique carbon source. The cellulose activity was 216 U L-1 in the medium with three carbon sources (glucose, carboxymethylcellulose and xylan), while xylanase activity was observed around the 200 h of fermentation and the highest value was 240 U L-1 in presence of the three carbon sources. Two isoforms of cellulase and xylanase and four laccase isoforms were observed in the zymograms. Most studies on the production of fungal enzymes in solid-state fermentation systems have been made using agroindustrial residues which acting as a support but also as a substrate so the quantification of biomass produced is complicated due to its complex composition, on the other hand is impossible determine whether enzymes of interest are constitutive or inducible. In this study, physiological parameters of Pleurotus ostreatus developed in solid culture could be obtained by using an inert support, also it was determined that in this fungus, laccases are constitutive enzymes, although activity values were changed depending on the carbon sources used, on the other hand, cellulases were inducible and susceptible to catabolite repression, while xylanase enzymes were observed in the three culture media, however, the highest activity value was produced in the culture medium with the three carbon sources.