Ibervillea sonorae callus cultures were established in order to produce fatty acids (lauric, myristic, pentadecanoic, palmitic and stearic acids) and phenolic compounds. Highest callus induction (100 %) was obtained in treatments containing 2.32 or 4.65 μM Kinetin (KIN) with 2.26 or 6.80 μM 2,4-Dichlorophenoxyacetic acid (2,4-D). Highest fatty acids (FA) production (48.57 mg g^-1), highest total phenol content (TPC; 57.1 mg gallic acid equivalents [GAE] g^-1and highest antioxidant activity (EC₅₀; 573.3-855.7 μg mL^-1) was obtained from callus derived from 4.65 μM KIN with 6.80 μM 2,4-D. A direct relationship was observed between the callus growth index (GI), FA content, TPC and EC₅₀. FA content was significantly higher in callus (48.57 mg g^-1) than the reported for tuber roots from wild plant (0.25 mg g^-1). TPC and EC₅₀ were higher in leaves (360.1 mg GAE g^-1 and 66.0-75.5 μg mL^-1, respectively) than in callus.