PRODUCTION OF BIOACTIVE COMPUNDS FROM IN VITRO CULTURES OF Tanacetum parthenium: PHENOLICS AND PARTHENOLIDE
Tanacetum parthenium has gained attention for drug development due to its production of bioactive compounds. In this work was evaluated the production of some of these bioactive compounds in organs of plantlets and root cultures. The plantlets were grown in culture medium free-plant growth regulators (PGRs) or treated with the PGRs α-naphtalenacetic acid (NAA) at 0.27 µM and kinetin (KIN) at 2.32 µM. Also, leaves removed from plantlets, were treated with 16.2 µM NAA to induce roots, following the establishment of root cultures. The shoots and roots of all plantlets, as well as the root cultures, produced phenolic compounds and parthenolide, although their content depended on the time of incubation. The highest content of phenolic compounds (total phenol content of 62.54 mg gallic acid equivalents [GAE] g-1; 4.261-4.973 mg of chlorogenic acid g-1; 0.858-1.015 mg of caffeic acid g-1; 1.931 mg of salicylic acid g-1) and parthenolide (0.071 mg g-1) were determined in shoots of plantlets under PGR treatment. Also, the PGRs enhanced morphogenesis and growth of plantlets, showing 13 shoots, 109 leaves, 97.4 mg dry weight (DW) of shoots, 51 mg DW of roots and a length of apical shoot of 7.4 cm per plantlet.
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