High pressure processing (HPP) and in-situ nisin biosynthesis by Lactococcus lactis: a hurdle approach to improve Listeria spp. inactivation in bovine milk

  • Carlos Regalado, Dr Universidad Autónoma de Querétaro http://orcid.org/0000-0002-3025-0313
  • Alejandra E. Alcántara-Zavala, MS DIPA, PROPAC, Universidad Autónoma de Querétaro
  • Vinicio Serment-Moreno, Dr Escuela de Ingeniería y Ciencias, Centro de Biotecnología FEMSA, Tecnológico de Monterrey
  • Karen I Velázquez-Lugo, MS DIPA, PROPAC, Universidad Autónoma de Queréato
  • Blanca E García-Almendárez, Dr DIPA, PROPAC, Universidad Autónoma de Querétaro
  • Jorge Welti-Chanes, Dr 2Escuela de Ingeniería y Ciencias, Centro de Biotecnología FEMSA, Tecnológico de Monterrey

Abstract

Raw milk cold pasteurization may be achieved by combining high pressure processing (HPP) with other (a)biotic factors. In this study, a combined approach involving HPP and biopreservation by in-situ nisin synthesis was evaluated. UHT and raw bovine milk were inoculated with Listeria innocua and treated with HPP (350-600 MPa), or HPP+nisin synthesized in-situ by Lactococcus lactis Rif L+ (LUQ2). HPP treatments of commercial UHT milk yielded two decimal reductions (SVHPP = 2 log10 reductions) of L. innocua with 550 MPa/3min, while the HPP+nisin approach yielded <10 cfu ml-1 under the same pressure-time conditions. Native Listeria monocytogenes was found in raw milk (~102 cfu ml-1) and the total Listeria spp. counts increased to ~109 cfu ml-1 with the L. innocua inoculum. HPP milk pasteurization (SVHPP ≥ 5 log10, aerobic plate count; <1 cfu ml-1, Listeria spp.) was viable by using 600 MPa/12 min, whereas at this pressure level the HPP+nisin approach resulted in milk pasteurization at holding times between 6 and 9 min. Furthermore, LUQ2 synthesized 9.75±0.54 IU ml-1 of nisin, which is below the FAO/WHO limit (500 IU g-1) suggesting that the promising HPP+nisin approach could be further optimized.

Published
Oct 29, 2017
How to Cite
REGALADO, Carlos et al. High pressure processing (HPP) and in-situ nisin biosynthesis by Lactococcus lactis: a hurdle approach to improve Listeria spp. inactivation in bovine milk. Revista Mexicana de Ingeniería Química, [S.l.], v. 17, n. 1, p. 269-277, oct. 2017. ISSN 2395-8472. Available at: <http://rmiq.org/ojs/index.php/rmiq/article/view/183>. Date accessed: 18 dec. 2017.
Section
Food Engineering

Received 4 July 2017, published online 29 October 2017