IN VIVO ANTI-INFLAMMATORY ACTIVITY AND ACUTE TOXICITY OF METHANOLIC EXTRACTS FROM WILD PLANT LEAVES AND CELL SUSPENSION CULTURES OF BUDDLEJA CORDATA KUNTH (BUDDLEJACEAE)

  • Gabriel Alfonso Gutiérrez-Rebolledo Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa
  • María Elena Estrada-Zúñiga Unidad de Investigación Médica en Farmacología, Hospital de Especialidades, CMN Siglo XXI, IMSS
  • Aurelio Nieto-Trujillo Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa
  • Francisco Cruz-Sosa Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa
  • M. Adelina Jiménez-Arellanes Universidad Autónoma del Estado de México

Abstract

Buddleja cordata is a species used by Mexican folk medicine for treating illnesses related to inflammation such as skin wounds and arthritis. It bio-synthesize metabolite such as verbascoside that contribute to its ethno-therapeutic properties as anti-inflammatory remedy. HPLC analysis showed that the methanolic extract from cell suspension cultures (Bc-Cc) and wild plant leaves (Bc-Wp) contained verbascoside, but concentration was higher in Bc-Cc (87.48 mg/g) than in wild plant (47.34 mg/g). In the acute toxicity model, none of the extracts generated any lethality or adverse effects. In acute inflammation model induced with TPA, Bc-Cc extract showed a greater edema inhibition at 2 mg/ear (61.72%), as well for carrageenan model at 200 mg/kg (48.87%). Bc-Wp showed lesser anti-inflammatory effect in both acute inflammation models than Bc-Cc. For Adjuvant-induced arthritis both extracts at 250 mg/kg generated a moderate inhibition over edema (≈33%) at day 28, and they were statistically no different to phenilbutazone. The culture in suspension of B. cordata obtained by biotechnological process contains greater amount of verbascosido and showed better anti-inflammatory activity; thus, representing a source for obtaining this type of secondary metabolite of pharmacological interest.

Published
Dec 16, 2017
How to Cite
GUTIÉRREZ-REBOLLEDO, Gabriel Alfonso et al. IN VIVO ANTI-INFLAMMATORY ACTIVITY AND ACUTE TOXICITY OF METHANOLIC EXTRACTS FROM WILD PLANT LEAVES AND CELL SUSPENSION CULTURES OF BUDDLEJA CORDATA KUNTH (BUDDLEJACEAE). Revista Mexicana de Ingeniería Química, [S.l.], v. 17, n. 1, p. 317-330, dec. 2017. ISSN 2395-8472. Available at: <http://rmiq.org/ojs/index.php/rmiq/article/view/256>. Date accessed: 16 jan. 2018.
Section
Biotechnology

Received 28 September 2017, published online 16 December 2017