IMPORTANCE OF DIFFUSSIVE FLUXES ON THE CARBON FATE WITH Aspergillus niger SURFACE CULTURES
This paper presents a simple experimental system to study surface cultures made of test tubes with height, L = 18.5 cm and internal diameter, D = 3.3 cm (A = 8.553 cm2). In this system, surface cultures of Aspergillus niger were supplied with 1.2 g total glucose but with dierent agar depths, H: 1.2, 2.3, 3.5 and 4.6 cm. Experimental variables were, surface uptake of glucose, biomass production, citric acid production and carbon dioxide as well as the final height of mycelia. The results show a positive linear correlation between S 0 and average glucose flux (JS ) as well as the respiratory rate, Rc. However, the flux of biomass (JX) and citric acid (JP) are negatively correlated to substrate initial concentration (S 0). The results suggest that carbon fate in these surface cultures depends on the magnitude of substrate influx to the fungal mat and the substrate uptake seems to be controlled by the rate of diusion of substrate of the medium to the surface of culture. Such results support the need to understand the importance of JS , in order to control carbon fate in fungal surface cultures.
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