• J.G. Monter-Miranda Centro de Investigación en Alimentación y Desarrollo, A.C.
  • J.M. Tirado-Gallegos Centro de Investigación en Alimentación y Desarrollo, A.C.
  • P.B. Zamudio-Flores Centro de Investigación en Alimentación y Desarrollo, A.C.
  • C. Rios-Velasco Centro de Investigación en Alimentación y Desarrollo, A.C.
  • J. de J. Ornelas-Paz Centro de Investigación en Alimentación y Desarrollo, A.C.
  • R. Salgado-Delgado Instituto Tecnológico de Zacatepec
  • V. Espinosa-Solis Universidad Autónoma de San Luis Potosí.
  • F. Hernández-Centeno Universidad Autónoma Agraria Antonio Narro
Keywords: insect, degree of deacetylation, molecular characterization, chitin, chitosan


Chitin was isolated from the insect Brachystola magna using an acid-alkali treatment (1 M of HCl and NaOH) at high temperature (97 °C); it was then deacetylated to chitosan (NaOH 40 % /110 °C/ 5.5 h). The dry weight yields of chitin and chitosan were 10.4 and 8.1% respectively. Chitin and chitosan were characterized by infrared spectroscopy with Fourier transform, X-ray diraction, scanning electron microscopy, potentiometric titration and high-performance size-exclusion chromatography; they were compared with commercial chitin and chitosan. The results showed that the obtained chitin and chitosan had chemical structures comparable to commercial polymers, with similar physicochemical properties; the obtained chitosan had a deacetylation degree of 87 % and high molecular weight (25.8 kDa). Brachystola magna Girard could be considered as a potential source for obtaining chitin and chitosan in significant quantities and at low cost.


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How to Cite
Monter-Miranda, J., Tirado-Gallegos, J., Zamudio-Flores, P., Rios-Velasco, C., Ornelas-Paz, J. de J., Salgado-Delgado, R., Espinosa-Solis, V., & Hernández-Centeno, F. (2019). EXTRACTION AND CHARACTERIZATION OF PHISICOCHEMICAL, MORPHOLOGICAL, AND STRUCTURAL PROPERTIES OF CHITIN AND CHITOSAN FROM Brachystola magna (Girard). Revista Mexicana De Ingeniería Química, 15(3), 749-761. Retrieved from http://rmiq.org/ojs311/index.php/rmiq/article/view/1028

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