Production and characterization of reconstituted strains of Pleurotus spp. cultivated on different agricultural wastes

  • J.D. Valenzuela-Cobos
  • A. Grijalva-Endara
  • R. Marcillo-Vallejo
  • M.F. Garcés-Moncayo
Keywords: Biodegradación, cepa reconstituida, Pleurotus, producción


Two reconstituted strains were produced by mating neohaplonts (monokaryons) obtained by chemical dedikaryotization of two parental strains i.e. Pleurotus ostreatus (OS) and Pleurotus djamor (DS). Five monokaryons were recovered employing homogenization time periods between 60 to 90 s, and incubation at 28oC in a peptone-glucose solution (PGS) for Pleurotus spp. Mycelial kinetics on MEA and wheat grain were determined using 4 math models, the parental strains on MEA showed μmax values between 8.11 and 10.73 mm day-1 and on wheat grain presented μmax values since 0.23 to 9.18 cm3 day-1, while the reconstituted strains on MEA exhibited μmax values ranged from 8.68 and 12.93 mm day-1 and on wheat grain showed μmax values between 0.25 and 9.30 cm3 day-1. The parental and reconstituted strains were cultivated using two agricultural wastes such as wheat straw (WS) and a mixture of oak sawdust, wheat straw, millet seed, cotton seed hull and CaCO3 (AP). The productivity and chemical composition of the mushrooms, and also the chemical composition of the substrates before and after harvest were determined. Reconstituted strain Pleurotus djamor (DS2xDS4) cultivated on WS showed the highest biological efficiency (125.84 %) and production rate (2.79 %), and also this strain produced on AP presented the highest productivity parameters: biological efficiency (98.43 %) and productivity rate (2.27 %). The reconstituted strain of Pleurotus ostreatus (OS4xOS5) produced on AP presented fruit bodies with highest protein content being of 24.03%, while the reconstituted strain of Pleurotus djamor (DS2xDS4) cultivated on WS exhibited the highest protein content of 32.94%. The substrate AP used in the cultivation of the reconstituted strain (DS2xDS4) presented the highest biodegradation value of lignin (33.60%).


Adebayo, A., Martínez-Carrera, D., Morales, P., Sobal, M., Escudero, H., Meneses, M., Avila-Nava, A., Castillo, I. and Bonilla, M. (2018). Comparative study of antioxidant and antibacterial properties of the edible mushrooms Pleurotus levis, P. ostreatus, P. pulmonarius and P. tuber-regium. International Journal of Food Science Technology 53, 1316-1330.
Aguilar-Doroteo, L., Zárate-Segura, P.B., Villanueva-Arce, R., Yánez-Fernández, J., Garín-Aguilar, M.E., Guadarrama-Mendoza, P.C. and Valencia del Toro, G. (2018). Utilización de marcadores ITS e ISSR para la caracterización molecular de cepas híbridas de Pleurotus djamor. Revista Iberoamericana de Micología 35, 49-55.
Alfaro M, Castanera R, Lavín JL, Grigoriev IV, Oguiza JA, Ramírez L, Pisabarro AG (2016) Comparative and transcriptional analysis of the predicted secretome in the lignocellulose-degrading basidiomycete fungus Pleurotus ostreatus. Environmental Microbiology 18, 4710–4726.
Aminuddin, H., Khan, A.M. and Madzlan, K. (2013). Effects of pH on mycelial growth and amino acid composition of Lentinula edodes in submerged cultures. Journal of tropical agriculture and food science 41, 63-70.
AOAC. (1997). Official Methods of Analysis. Association of Official Analytical Chemists. 16th ed. Whashington U.S.A.
Bae, J.S., Kim, Y.I., Jung, S.H., Oh, Y.G. and, Kwak, W.S. (2006). Evaluation on feed-nutritional value of spent mushroom (Pleurotus osteratus, Pleurotus eryngii, Flammulina velutupes) substrates as a roughage source of ruminants. Journal of Animal Science and Technology 48, 237–246.
Blanchette, R.A. (1991) Delignification by wood-decay fungi. Annual Review of Phytopathology 29, 381–398.
Baty, F. and Delignette-Muller, M.L. (2004). Estimating the bacterial lag time: which model, which precision? International Journal of Food Microbiology 91, 261-277.
Carroad, P.A. and Wilde, C.R. (1977). Exponential growth kinetics for Polyporus versicolor and Pleurotus ostreatus in submerged culture. Applied and Environmental Microbiology 33, 871-873.
Clark, T.A. and Anderson, J.B. (2004). Dikaryons of the basidiomycete fungus Schizophyllum commune: evolution in longterm culture. Genetics 167, 1663–75
Chatterjee, T., Chatterjee, B.K., Majumdar, D. and Chakrabarti, P. (2015). Antibacterial effect of silver nanoparticles and the modeling of bacterial growth kinetics using a modified Gompertz model. Biochimica et Biophysica Acta 1850, 299-306.
Crisan, E.V. and Sands, A. (1978). Nutritional value. In: The Biology and Cultivation of Edible Mushroom (S.T.Chang, & W.A. Hayes, eds.), Pp. 137-165. Academic Press Inc, USA.
Gaitán-Hernández, R., Esqueda, M., Gutiérrez, A., Sánchez, A., Beltrán-García, M. and Mata, G. (2006). Bioconversion of agrowastes by Lentinula edodes: the high potential of viticulture residues. Applied Microbiology and Biotechnology 71, 432-439.
Gibson, A.M., Bratchell, N. and Roberts, T.A. (1987). The effect of sodium chloride and temperature on the rate and extent of growth of Clostridium botulinum type A in pasteurized pork slurry. Journal of Applied Microbiology 62, 479-490.
Guadarrama-Mendoza, P.C., Valencia del Toro, G., Ramírez-Carrillo, R., Robles-Martinez, F., Yáñez-Fernández, J., Garín-Aguilar, M.E. and Bravo-Villa, G. (2014). Morphology and mycelial growth rate of Pleurotus spp. strains from the Mexican mixtec region. Brazilian Journal of Microbiology 45, 861-872
Guillén-Navarro, G.K., Márquez-Rocha, F.J. and Sánchez-Vázquez, J.E. (1998). Producción de biomasa y enzimas ligninolíticas por Pleurotus ostreatus en cultivo sumergido. Revista Iberoamericana de Micología 15, 302-306
Iossi, M., Valenzuela Cobos, J.D., Gea, F.J. and Zied, D. (2018). Pleurotus spp. cultivation on Brachiaria sp. straw treatment with alkaline water. Brazilian Journal of Microbiology 49, 64-67.
Kawasumi, T., Kiuchi, N., Futatsugi, Y., Kazuhiro, O.H.B. and Yanagi, S.O. (1987). High yield preparation of Lentinus edodes (“Shiitake”) protoplasts with regeneration capacity and mating type stability. Agricultural and Biological Chemistry 51, 1649-1656.
Kim, S.W., Hwang, H.J., Park, J.P., Cho, Y.J., Song, C.H. and Yun, J.W. (2002). Mycelial growth and exo‐biopolymer production by submerged culture of various edible mushrooms under different media. Letters in Applied Microbiology 34, 56-61.
Leal-Lara, H. and Eger-Hummel, G. (1982). A monokaryotization method its use for genetic studies in wood-rooting basidiomycetes. Theoretical and Applied Genetics 61, 65-68.
Liu, H., Chen, N., Feng, C., Tong, S. and Li, R. (2017). Impact of electrostimulation on denitrifying bacterial growth and analysis of bacterial growth kinetics using a modified Gompertz model in a bio-electrochemical denitrification reactor. Bioresource Technology 232, 344-353
López, S., Prieto, M., Dijkstra, J., Dhanoa, M.S. and France, J. (2004). Statistical evaluation of mathematical models for microbial growth. International Journal of Food Microbiology 96, 289-300
Loshchinina, E.A. and Nikitina, V.E. (2016) Role of the NO synthase system in response to abiotic stress factors for Basidiomycetes Lentinula edodes and Grifola frondosa. Microbiology 85,165–171
Mandeel, Q.A., Al-Laith, A.A. and Mohamed, S.A. (2005) Cultivation of oyster mushrooms (Pleurotus spp.) on various lignocellulosic wastes. World Journal of Microbiology and Biotechnology 4, 601–607.
Manzi, P., Aguzzi, A. and Pizzoferrato, L. (2001). Nutritional value of mushrooms widely consumed in Italy. Food Chemistry 73, 321-325.
Oropeza-Guerrero, M.P., Santos-Sánchez, N.F., Salas-Coronado, R., Valadez-Blanco, R., Hernández-Carlos, B. and Guadarrama-Mendoza, P.C. (2018). Productivity and Antioxidant Activity of Wild, Reconstituted, and Hybrid Strains of the Pink Oyster Mushroom, Pleurotus djamor (Agaricomycetes), from Mexico. International Journal of Medicinal Mushrooms 20, 607-621
Reis, F.S., Barros, L., Martins, A. and Ferreira, I. (2012). Chemical composition and nutritional value of the most widely appreciated cultivated mushrooms: An inter-species comparative study. Food and Chemical Toxicology 50, 191-197.
Sánchez, C. (2009) Lignocellulosic residues: biodegradation and bioconversion by fungi. Biotechnology Advances 27, 185–194.
Sánchez-Hernández, A., Valenzuela Cobos, J.D., Herrera Martínez, J., Villanueva Arce, R., Gómez y Gómez, Y.M., Zarate Segura, P.B., Garín Aguilar, M.E., Leal Lara, H. and Valencia del Toro, G. (2019). Characterization of Pleurotus djamor neohaplonts recovered by production of protoplasts and chemical dedikaryotization. 3 Biotech 9:24
Salmones, D., Gaitán-Hernández, R., Pérez, R. and Guzmán, G. (1997) Estudios sobre el género Pleurotus VIII. Interacción entre crecimiento micelial y productividad. Revista Iberoamericana de Micología 14, 173-176
Salmones, D., Mestizo, L. and Gaitán–Hernández, R. (2004). Entrecruzamiento y evaluación de la producción de las variedades de Pleurotus djamor (Fr.) Boedijn. Revista Mexicana de Micología 18, 21–26.
Valencia del Toro, G. and Leal-Lara, H. (1999). Estudios de compatibilidad entre cepas de Pleurotus spp con cuerpos fructíferos de diversos colores. Revista Mexicana de Micología 15, 65-71.
Valencia del Toro, G., Ramírez-Ortiz, M.E., Flores-Ramírez, G., Costa-Manzano, M.R., Robles-Martínez, F., Garín Aguilar, M.E. and Leal-Lara, H. (2018). Effect of Yucca schidigera bagasse as substrate for Oyster mushroom on cultivation parameters and fruit body quality. Revista Mexicana de Ingeniería Química 17, 835-846.
Valenzuela-Cobos, J.D., Durán Páramo, E., Villanueva Arce, R., Sánchez Hernández, A., Garín Aguilar, M.E., Leal Lara, H. and Valencia del Toro, G. (2017). Production of hybrid strains among Pleutorus and Lentinula and evaluation of their mycelial growth kinetics on malt extract agar and wheat grain using the Gompertz and Hill models. Emirates Journal of Food and Agriculture 29, 927-935.
Valenzuela-Cobos, J.D., Vásquez-Véliz, G.K., Zied, D.C., Franco-Hernández, O.M., Sánchez Hernández, A., Garín Aguilar, M.E., Leal Lara, H. and Valencia del Toro, G. (2019). Bioconversion of agricultural wastes using parental, hybrid and reconstituted strains of Pleurotus and Lentinula. Revista Mexicana de Ingeniería Química 18, 647-657.
Zwietering, M.H., Jongenburger, I., Rombouts, F.M. and Van’triet, K. (1990). Modeling of the bacterial growth curve. Applied Microbiology and Biotechnology 56, 1875– 188.
How to Cite
Valenzuela-Cobos, J., Grijalva-Endara, A., Marcillo-Vallejo, R., & Garcés-Moncayo, M. (2020). Production and characterization of reconstituted strains of Pleurotus spp. cultivated on different agricultural wastes. Revista Mexicana De Ingeniería Química, 19(3), 1493-1504.

Most read articles by the same author(s)