• A.I. Reyes-Mendez Instituto Politécnico Nacional
  • C. Figueroa-Hernández Instituto Politécnico Nacional
  • G. Melgar-Lalanne Instituto Politécnico Nacional
  • H. Hernández-Sánchez Instituto Politécnico Nacional
  • G. Davila-Ortiz Instituto Politécnico Nacional
  • C. Jiménez-Martínez Instituto Politécnico Nacional
Keywords: bioactive peptides, Bacillus subtilis, Bacillus clausii, Bacillus coagulans, calcium-binding, iron-binding, proteolysis, spirulina


Some strains of Bacillus subtilis, B. clausii and B. coagulans are commercially used as probiotic bacteria and their proteolytic activity is well known. The aim of this work was to evaluate their capacity to produce calcium- and ironbinding peptides when grown in culture media with different nitrogen sources. The fermentation media included broths containing tryptic soy (TS), casein peptone (CP), soy peptone (SP), and a crude phycobiliprotein extract (CPE). Cell-free supernatants (CFS) were prepared from the fermented media after 24, 48 and 72 h of fermentation and, tested for degree of hydrolysis (DH). Calcium- and iron-binding activities were determined as well. When the inocula were prepared in a medium designed for the production of alkaline proteases (AP), the degree of hydrolysis and the mineral-binding activity in the CFS were higher. The best results for calcium-binding activity and DH were obtained when B. subtilis and B. coagulans grew in a CPE-containing medium. Analogous results were found for the iron-binding activity; nevertheless, this bioactivity was also high for B. clausii when grown in broths containing TS-, CP- and, SP.


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How to Cite
Reyes-Mendez, A., Figueroa-Hernández, C., Melgar-Lalanne, G., Hernández-Sánchez, H., Davila-Ortiz, G., & Jiménez-Martínez, C. (2020). PRODUCTION OF CALCIUM- AND IRON-BINDING PEPTIDES BY PROBIOTIC STRAINS OF Bacillus subtilis, B. clausii AND B. coagulans GBI-30. Revista Mexicana De Ingeniería Química, 14(1), 1-9. Retrieved from

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