CHITOSAN/CARRAGEENAN/LYSOZYME PARTICLES: SYNTHESIS, CHARACTERIZATION AND ANTIFUNGAL ACTIVITY AGAINST Aspergillus parasiticus

  • C.N. Hernández-Téllez
  • M.O. Cortez-Rocha
  • A. Burgos Hernández
  • E.C. Rosas-Burgos
  • J. Lizardi-Mendoza
  • W. Torres-Arreola
  • M.G. Burboa-Zazueta
  • M. Plascencia-Jatomea
Keywords: carrageenan, biocomposites, subcellular structures, cytotoxicity, protein release

Abstract

In this study, particles of chitosan-carrageenan (Cs-Crg) and chitosan-carrageenan-lysozyme (CS-Crg-LZ) were synthesized by ionic complexation. The particles were analyzed by atomic force microscopy, dynamic light scattering and FT-IR, and the percentage of association of lysozyme was determined. The biological activity of the particles was evaluated by viability assays of Aspergillus parasiticus spores and cytotoxicity in ARPE-19 cells. The effect on subcellular structures was analyzed by fluorescence microscopy. The addition of lysozyme reduced the size of the particles. The size and Z potential of the synthesized particles were 725±5.5 and 250±8.1 nm and 28.7±1.0 and 21.6±0.8 mV for CS-Crg and CS-Crg-LZ, respectively. CS-Crg-LZ and CS-Crg particles exhibited a higher (p<0.05) inhibitory effect on the viability of A. parasiticus with respect to control, which increased proportionally to the particles concentration. None of the particles showed a mutagenic effect and they did not present cytotoxicity in ARPE-19 cells; likewise, no changes were observed in cell morphology with respect to control, observing normal structures in the actin cytoskeleton and in the DNA structure. These results show that chitosan-based particles are a feasible and safe option for the control of pathogenic fungi of agricultural-food interest.

Published
2018-06-03
How to Cite
Hernández-Téllez, C., Cortez-Rocha, M., Burgos Hernández, A., Rosas-Burgos, E., Lizardi-Mendoza, J., Torres-Arreola, W., Burboa-Zazueta, M., & Plascencia-Jatomea, M. (2018). CHITOSAN/CARRAGEENAN/LYSOZYME PARTICLES: SYNTHESIS, CHARACTERIZATION AND ANTIFUNGAL ACTIVITY AGAINST Aspergillus parasiticus. Revista Mexicana De Ingeniería Química, 17(3), 897-912. Retrieved from http://rmiq.org/ojs311/index.php/rmiq/article/view/12

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