• A.S. Pérez-Martínez División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica A.C
  • A.P. Barba de la Rosa División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica A.C
  • A. De León-Rodríguez División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica A.C
Keywords: chitin, cell size, recombinant endochitinase, oxygen-limited fermentation


The expression of recombinant Trichoderma atroviride endochitinase ech42 (rech42) in Pichia pastoris driven by the constitutive PGAP promoter was tested at low (3%) and high (40%) dissolved oxygen tension (DOT) conditions. Maximum volumetric enzyme activities of 18,600±1053 and 12,330±1000 U l−1 were attained at 3% and 40% DOT, respectively. Whereas maximum specific enzyme activities were 89.4±4.6 y 88.8±4.0 U mg−1 protein. We found that conjunction of low DOT and the overexpression of rech42 affected the cell size of the P. pastoris but no the rech42 specific enzyme activity


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How to Cite
Pérez-Martínez, A., Barba de la Rosa, A., & De León-Rodríguez, A. (2020). HETEROLOGOUS EXPRESSION OF Trichoderma atroviride ENDOCHITINASE ech42 IN Pichia pastoris AT LOW AND HIGH DISSOLVED OXYGEN TENSIONS. Revista Mexicana De Ingeniería Química, 13(1), 93-101. Retrieved from http://rmiq.org/ojs311/index.php/rmiq/article/view/1300