INSIGHT OVER Lactobacillus plantarum 299V PHYSICOHEMICAL CHARACTERISTICS OF AGGREGATION KINETICS UNDER STARVATION AND DIFFERENT PH CONDITIONS

Keywords: probiotics, cell surface properties, zeta potential, image analysis, aggregation

Abstract

Bacterial aggregation and adhesion are ruled by specific and nonspecific adhesion mechanisms. In the case of the probiotic strain Lactobacillus plantarum 299v (Lp299v) the biochemistry of adhesion has been previously studied and s-layer proteins have been identified. However, physic-chemical mechanisms which ruled the aggregation and adhesion phenomena able to change through the time under different pH conditions have not been completely elucidated.

The non-specific adhesion mechanisms (hydrophobicity, zeta potential and morphology) of Lp299v auto-aggregates over time (24 h) at different pH levels (from 5.0 to 9.0) on starvation were described. Auto-aggregation resulted in greater dependency on time. The Lp299v cell surface showed a higher level of affinity with chloroform than n-hexadecane, while the zeta potential was a negative charge in all cases. Size and shape parameters were found to be dependent on time and pH. A strong Pearson correlation (0.991) was observed between circularity and auto-aggregation. In conclusion, Lp299v’s nonspecific adhesion mechanism was found to be more related to time than pH levels, indicating that Van der Waals and hydrophobic forces and shape parameters have the potential to dominate the autoaggregation of Lp299v. However, the predictive model presented shows that lower pH may promote auto-aggregation and thus facilitate the colonization at the upper level of the digestive system.

Author Biographies

G. Melgar-Lalanne

Instituto de Ciencias Básicas. Universidad Veracruzana. Av. Dr. Luis Castelazo Ayala s/n. Col Industrial Ánimas, 91192. Xalapa, Veracruz, Mexico

J. Ley-Martinez

Instituto de Ciencias Básicas. Universidad Veracruzana. Avda. Dr. Luis Castelazo Ayala s/n. Col. Industrial Ánimas. 91192. Xalapa, Ver. México

E. Azuara-Nieto

Instituto de Ciencias Básicas. Universidad Veracruzana. Av. Dr. Luis Castelazo Anaya s/n. Col. Industrial Animas. 91192. Xalapa, Ver. Mexico

D.I. Tellez-Medina

Escuela Nacional de Ciencias Biológicas. Unidad Profesional Adolfo López Mateos. Av. Wilfrido Massieu Es. Cda. Miguel Stampa s7n. Col Zacatenco. Cp. 07738. Ciudad de Mexico. Mexico.

C.R. González-González

Instituto Tecnológico Superior de Acayucan. Carretera Costera del Golfo km 216.4. Colonia Agricola Michapa. Cp 96100. Acayucan. Veracruz

G.F. Gutierrez-Lopez

Escuela Nacional de Ciencias Biológicas. Unidad Profesional Adolfo López Mateos, Av. Wilfrido Massieu Esq. Cda. Miguel Stampa s/n, Col. Zacatenco, C.P.07738. Delegación Gustavo A. Madero. Mexico City. Mexico

T. Meza

Laboratorio de Genómica Humana, Facultad de Medicina, Universidad Veracruzana, Calle Médicos y Odontólogos S/N., Col. Unidad del Bosque, C. P. 91010, Xalapa, Veracruz, Mexico.

Published
2018-09-12
How to Cite
Melgar-Lalanne, G., Ley-Martinez, J., Azuara-Nieto, E., Tellez-Medina, D., González-González, C., Gutierrez-Lopez, G., & Meza, T. (2018). INSIGHT OVER Lactobacillus plantarum 299V PHYSICOHEMICAL CHARACTERISTICS OF AGGREGATION KINETICS UNDER STARVATION AND DIFFERENT PH CONDITIONS. Revista Mexicana De Ingeniería Química, 18(1), 151-164. Retrieved from http://rmiq.org/ojs311/index.php/rmiq/article/view/137
Section
Biotechnology

Most read articles by the same author(s)