• R.I. Corona-González
  • J.R. Ramos-Ibarra
  • P. Gutiérrez-González
  • C. Pelayo-Ortiz
  • G.M. Guatemala-Morales
  • E. Arriola-Guevara
Keywords: tepache, fermentation,, response surface methodology, hedonic scale, yeast identification


Due to the artisan nature of the production process of tepache, there is no uniformity in fermentation conditions and raw material used. The fermentation is crucial to the final characteristics of tepache and there is a certain degree of ignorance of related organisms and the level of acceptance among consumers. In this study, tepache fermentation conditions (concentration of sugars, initial pH, temperature and fermentation time) were evaluated to enable correlating the formation of products of fermentation (lactic, acetic and ethanol) with the degree of acceptance of the beverage among consumers. Sensory evaluation was measured on a 9-point hedonic scale. Results were analyzed using the response surface methodology (RSM) which showed that the fermentation conditions for higher acceptance were: 22oC, 10% (mass/volume) of sugars (brown sugar), 72 h of fermentation and an initial pH of 5. According to this study, to have a wider acceptance the beverage must contain about 7 g/L of ethanol, no more than 5 g/L of lactic and acetic acid, and 70 g of sucrose/L. Likewise, yeasts present in the fermentation were identified and it was found that Saccharomyces cerevisiae is the predominant species


Aidoo, K., Rob, M. and Sarkar, P. (2006). Occurrence and function of yeasts in Asian indigenous fermented foods. FEMS Yeast Research 6, 30-39.

Alvarado, C., García, B., Martin, S. and Regalado, C. (2006). Food-associated lactic acid bacteria with antimicrobial potential from traditional Mexican foods. Revista Latinoamericana de Microbiología 48, 260-268.

Benda, I. (1984). Wine and Brandy. In: Prescott & Dunn's industrial microbiology, (4th edn) (G. Reed ed.), Pp 293-402. Avi publishing company, Connecticut. USA.

Chanprasartsuk, O., Prakitchaiwattana, C., Sanguandeekul, R. and Fleet, G. (2010). Autochthonous yeasts associated with mature pineapple fruits, freshly crushed juice and their ferments and the chemical changes during natural fermentation. Bioresource Technology 101, 7500-7509.

Deshpande, R., Chinnan, M. and Mc Watters, K. (2008). Optimization of a chocolate flavored, peanut-soy beverage using response surface methodology (RSM) as applied to consumer acceptability data. LWT Food Science Technology 4, 1485-1492.

Duarte,W. F., Amorim, J. C., de Assis Lago, L., Dias, D. R, and Schwan, R. (2011). Optimization of Fermentation Conditions for Production of the Jabuticaba (Myrciariacauliflora) Spirit Using the Response Surface Methodology. Journal of Food Science 76, C782-C790.

Escalante, A., Rodríguez, M., Martínez, A.,López-Munguía, A., Bolívar, F. and Gosset, G. (2004). Characterization of bacterial diversity in pulque, a traditional Mexican alcoholic fermented beverage, as determined by 16S rDNA analysis. FEMS Microbiology Letters 235, 273-279.

Estela-Escalante, W., Rychtera, M., Melzoch, K. and Hatta-Sakoda, B. (2012). Effect of aeration on the fermentative activity of Saccharomyces cerevisiae cultured in apple juice. Revista Mexicana de Ingeniería Química 11, 211-226.

Esteve-Zarzoso, B., Belloch, C., Uruburu, F. and Querol, A. (1999). Identification of yeast by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. International Journal of Systematic Bacteriology 49, 329-337.

Flores-Ramírez, N., Martínez-Peniche, R. A., Fernández-Escartín, E., Gallegos-Pérez, J. L., Díaz-Cervantes, M. I and Vásquez-García, S. R. (2005). Physical and chemical properties in red wines: traditional vinification and carbonic maceration with two Saccharomyces cerevisiae strains. Revista Mexicana de Ingeniería Química 4, 289-297.

Garde, T. and Ancín, C. (2006). Contribution of wild yeasts to the formation of volatile compounds in inoculated wine fermentations. European Food Research and Technology 222, 15-25.

Godoy, A., Herrera, T. and Ulloa, M. (2003). Más allá del pulque y el tepache. UNAM, México.

González-Hernández, J. C., Pérez, E., Damián, R. M. and Chávez, M. C. (2012). Isolation, molecular and fermentative characterization of a yeast used in ethanol production during mezcal elaboration. Revista Mexicana de Ingeniería Química 11, 389-400.

Lincoln, L. (1985). Regresión y correlación. In: Introducción a la estadística, (L. Lincoln ed.), Pp 399-424. CECSA, México.

Manca, M., Strasser, A. and Arena, M. (2006). Bebidas fermentadas. In: Microbiología de los alimentos, (M. Torres, A. Castillo, eds.), Pp 371-392. Universidad de Guadalajara, México. Montgomery, D. (2010). Disen˜o y Análisis de Experimentos. Limusa Wiley, México.

Moreno-Terrazas, R. D. (2005). Determinación de las características microbiológicas, bioquímicas, fisicoquímicas y sensoriales para la estandarización del proceso de la elaboración del tepache. [PhD thesis]. Iztapalapa, México: Universidad Autónoma Metropolitana. Available from: presentatesis.php?recno=11992&docs=UAMI 11992.PDF. Accessed 19 August 2011.

Nwabueze, T. U. (2010). Review article: Basic steps in adapting response surface methodology as mathematical modeling for bioprocess optimization in the food systems. International Journal of Food Science Technology 45, 1768- 1776.

Prasad, K. and Nath, N. (2002). Comparison of sugarcane juice based beverage optimization using response surface methodology with Fuzzy method. Sugar Technology 4, 109-115.

Querol, A., Barrio, E., Huerta, T. and Ramón,D. (1992). Molecular monitoring of wine fermentations conducted by active dry yeast strains. Applied Environmental Microbiology 58, 2948-2953.

Ratman, B., Narasimha, M., Damodar, M., Subba,S. and Ayyanna, C. (2003). Optimization of fermentation conditions for the production of ethanol from sago starch using response surface methodology. World Journal of Microbiology and Biotechnology 19, 523-526.

Ratman, B., Subba, S., Damodar, M., Narasimha, M. and Ayyanna, C. (2005). Optimization of medium constituents and fermentation conditions for the production of ethanol from palmyra jaggery using response surface methodology. World Journal of Microbiology and Biotechnology 21, 399-404.

Romano, P., Capece, A. and Jespersen, L. (2006). Taxonomic and ecological diversity of food and beverage yeasts. In: Yeasts in Food and Beverages, (A. Querol, G. Fleet eds.), Springer, New York. USA.

Scragg, A. (2009). Biotecnología para ingenieros: Sistemas biológicos en procesos tecnológicos. Limusa, México. StatPoint Tecnology, Inc (2005). Statgraphics Centurion (Data Analysis Software System), v. XV, Warrenton, VA (

Suder, Y., Prakasam, R. S. and Reddy, O. V. S. (2009). Optimization of fermentation conditions for mango (Mangiferaindica L.) wine production by employing response surface. International Journal of Food Science Technology 44, 2320-2327

Swiers, J., Bartowsky, E., Henschke, P. and Pretorius, I. (2005). Yeast and bacterial modulation of wine aroma and flavor. Australian Journal of Grape Wine Research 11, 139-173.

Téllez-Mora, P., Peraza-Luna, F. A., Feria-Velasco, A. and Andrade-González, I. (2012). Optimization of fermentation process for tequila production using response surface methodology (RSM). Revista Mexicana de Ingeniería Química 11, 163-176.

Valim, M. F., Rossi, E. A., Silva, R. S. and Borsato, D. (2003). Sensory acceptance of a functional beverage based on orange juice and soy milk. Brazilian Journal of Food Technology 6, 153-156
How to Cite
Corona-González, R., Ramos-Ibarra, J., Gutiérrez-González, P., Pelayo-Ortiz, C., Guatemala-Morales, G., & Arriola-Guevara, E. (2020). THE USE OF RESPONSE SURFACE METHODOLOGY TO EVALUATE THE FERMENTATION CONDITIONS IN THE PRODUCTION OF TEPACHE. Revista Mexicana De Ingeniería Química, 12(1), 19-28. Retrieved from
Food Engineering

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