Biofunctional characterization of red, black and white ginseng (Panax ginseng Meyer) root extracts

  • F. Hussain
  • A. Akram
  • J. Hafeez
  • M. Shahid
Keywords: P. ginseng, antioxidant, enzyme inhibition, antidiabetic, phenols, biofilm.

Abstract

An organized biofunctional characterization of red, black, and white ginseng root extracts and fractions was performed. To assess antioxidant, antiglycation, enzyme inhibitory, antiamnestic, cytotoxic, thrombolytic, antibiofilm potentials in roots of three Panax ginseng preparations (red, black, white), powdered material was extracted and fractionated into different solvents. Antioxidant activity, phenolic and flavonoids contents were assessed by using free radical scavenging, Folin–Ciocalteu reagent and aluminum chloride colorimetric assays. Antiglycation and enzyme (alpha amylase, alpha glucosidase and acetylcholinesterse) inhibition activities were tested by prescribed methods along with cytotoxic (antihemolytic profile), thrombolytic activity, and biofilm growth inhibition assays. Red and black ginseng indicated maximum phenolic contents (37.26 ± 1.563 g GAE) and flavonoid contents (149.4 ± 2.032 g CE) respectively. The DPPH scavenging ability (62.84%) of red ginseng was higher than black and white ginseng. Significant antidiabetic activities were exhibited by black and white ginseng. The highest antihemolytic (71.2%) and thrombolytic (87%) actions were shown by black and red ginseng respectively. Red and white ginseng maximally inhibited the growth of Pasteurella multocida while in the case of Staphylococcus aureus, red ginseng showed optimum antibiofilm activity. The present study demonstrates that all three preparations of P. ginseng have effective yet variable functional characteristics that warrant further exploration.

References

Ahn, H.Y., Hong, S.Y., Kim, J.Y. and Kwon, O. (2013). Panax ginseng extract rich in ginsenoside protopanaxatriol offers combinatorial effects in nitric oxide production via multiple signaling pathways. Springerplus 2, 96. https:// doi: 10.1186/2193-1801-2-96
Ali, M.S., Amin, M.R., Kamal, C.M.I. and Hossain, M.A. (2013). In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves. Asian Pacific Journal of Tropical Biomedicine 3, 464-69. https:// doi: 10.1016/S2221-1691(13)60097-0
Apostolidis, E., Kwon, Y.I., Shetty, K., Apostolidis, E. and Kwon, Y.I. (2006). Potential of cranberry-based herbal synergies for diabetes and hypertension management. Asia Pacific Journal of Clinical Nutrition 15, 433-41.
Chahardehi, A.M., Ibrahim, D. and Sulaiman, S.F. (2009). Antioxidant activity and total phenolic content of some medicinal plants in Urticaceae family. Journal of Applied Biological Sciences 3, 27-31.
Chen, X.P., Lin, Y.P., Hu, Y.Z., Liu, C.X., Lan, K. and Jia, W. (2015). Phytochemistry, metabolism, and metabolomics of ginseng. Chinese Herbal Medicines, 7, 98-108. https://doi.org/10.1016/S1674-6384(15)60026-0
Chen, W., Balan, P. and Popovich, D.G. (2019). Review of ginseng anti-diabetic studies. Molecules 24, 4501. https:// doi:10.3390/molecules24244501
Chung, I.M., Lim, J.J., Ahn, M.S., Jeong, H.N., An, T.J. and Kim, S.H. (2015). Comparative phenolic compound profiles and antioxidative activity of the fruit, leaves, and roots of Korean ginseng (Panax ginseng Meyer) according to cultivation years. Journal of Ginseng Research 40, 68-75. https://doi: 10.1016/j.jgr.2015.05.006
Dhama, K., Tiwari, R., Chakraborty, S., Saminathan, M., Kumar, A., Karthik, K., Wani, M.Y., Amarpal, S.S. and Rahal, A. (2014). Evidence based antibacterial potentials of medicinal plants and herbs countering bacterial pathogens especially in the era of emerging drug resistance: An integrated update. International Journal of Pharmacology 10, 1-43. https://doi: 10.3923/ijp.2014.1.43
Dheepa, M., Vinitha, L.R. and Appalaraju, B. (2011). Comparison of biofilm production and multiple drug resistance in clinical isolates of Acinetobacter baumanii from a tertiary care hospital in South India. International Journal Pharmaceutical and Biomedical Sciences 2, 103-7
Dhawan, V. and Jain, S. (2005). Garlic supplementation prevents oxidative DNA damage in essential hypertension. Molecular and Cellular Biochemistry 275, 85-94. https://doi: 10.1007/s11010-005-0824-2.
Han, M.J. and Kim, D.H. (2020). Effects of red and fermented ginseng and ginsenosides on allergic disorders. Biomolecules 10, 634. https://doi:10.3390/biom10040634
Hossain, M.S., Chowdhury, M.E.H., Das, S. and Chowdhury, I.U. (2012). In vitro thrombolytic and anti-inflammatory activity of Swertia chirata ethanolic extract. Journal of Pharmacognosy and Phytochemistry 1, 99-104.
Iqbal, H. and Rhee, D.K. (2020). Ginseng alleviates microbial infections of the respiratory tract: a review. Journal of Ginseng Research 44, 194-204. https://doi: 10.1016/j.jgr.2019.12.001.
Jakaria, M., Kim, J., Karthivashan, G., Park, S.Y., Ganesan, P. and Choi, D.K. (2019). Emerging signals modulating potential of ginseng and its active compounds focusing on neurodegenerative diseases. Journal of Ginseng Research 43, 163-71. https://doi.org/10.1016/j.jgr.2018.01.001
Jin, Y.R., Yu, J.Y., Lee, J.J., You, S.H., Chung, J.H., Noh, J.Y., Im, J.H., Han, X.H., Kim, T.J., Shin, K.S. and Wee, J.J. (2007). Antithrombotic and antiplatelet activities of Korean red ginseng extract. Basic & Clinical Pharmacology & Toxicology 100, 170-75. https://doi: 10.1111/j.1742-7843.2006.00033.x.
Jin, Y., Kim, Y.J., Jeon, J.N., Wang, C., Min, J.W., Noh, H.Y. and Yang, D.C. (2015). Effect of white, red and black ginseng on physicochemical properties and ginsenosides. Plant Foods for Human Nutrition 70, 141-45. https://doi:10.1007/s11130-015-0470-0
Jin, Y., Cui, R., Zhao, L., Fan, J. and Li, B. (2019). Mechanisms of Panax ginseng action as an antidepressant. Cell Proliferation 52, p.e12696. https:// doi:10.1111/cpr.12696
Jung, C.H., Seog, H.M., Choi, I.W., Choi, H.D. and Cho, H.Y. (2005). Effects of wild ginseng (Panax ginseng CA Meyer) leaves on lipid peroxidation levels and antioxidant enzyme activities in streptozotocin diabetic rats. Journal of Ethnopharmacology 98, 245-50. https:// doi: 10.1016/j.jep.2004.12.030.
Kim, J.S. (2016). Investigation of phenolic, flavonoid, and vitamin contents in different parts of Korean Ginseng (Panax ginseng CA Meyer). Preventive Nutrition and Food Science 21, 263-70. https://doi: 10.3746/pnf.2016.21.3.263
Kim, K.H., Lee, D., Lee, H.L., Kim, C.E., Jung, K. and Kang, K.S. (2018). Beneficial effects of Panax ginseng for the treatment and prevention of neurodegenerative diseases: past findings and future directions. Journal of Ginseng Research 42, 239-47. https://doi.org/10.1016/j.jgr.2017.03.011
Kwon, H.W., Shin, J.H., Cho, H.J., Rhee, M.H. and Park, H.J. (2016). Total saponin from Korean Red Ginseng inhibits binding of adhesive proteins to glycoprotein IIb/IIIa via phosphorylation of VASP (Ser157) and dephosphorylation of PI3K and Akt. Journal of Ginseng Research 40, 76-85. https://doi: 10.1016/j.jgr.2015.05.004
Lee, M.R., Yun, B.S. and Sung, C.K. (2012). Comparative study of white and steamed black Panax ginseng, P. quinquefolium, and P. notoginseng on cholinesterase inhibitory and antioxidative activity. Journal of Ginseng Research 36, 93-101. https://doi: 10.5142/jgr.2012.36.1.93
Lee, D., Ghafoor, K., Moon, S., Kim, S.H., Kim, S., Chun, H. and Park, J. (2015). Phenolic compounds and antioxidant properties of high hydrostatic pressure and conventionally treated ginseng (Panax ginseng) products. Quality Assurance and Safety of Crops & Foods 7, 493-500.
Li, G.X. and Liu, Z.Q. (2008). The protective effects of ginsenosides on human erythrocytes against hemin-induced hemolysis. Food and Chemical Toxicology 46, 886-92. https://doi:10.1016/j.fct.2007.10.020
Liu, Z.Q., Luo, X.Y., Sun, Y.X., Chen, Y.P. and Wang, Z.C. (2002). Can ginsenosides protect human erythrocytes against free-radical-induced hemolysis?. Biochimica et Biophysica Acta (BBA)-General Subjects 1572, 58-66. https://doi: 10.1016/s0304-4165(02)00281-7.

Matsuda, H., Wang, T., Managi, H. and Yoshikawa, M. (2003). Structural requirements of flavonoids for inhibition of protein glycation and radical scavenging activities. Bioorganic & Medicinal Chemistry 11, 5317-23.https://doi.org/10.1016/j.bmc.2003.09.045
Mehmood, N., Zubaır, M., Rızwan, K., Rasool, N., Shahid, M. and Ahmad, V.U. (2012). Antioxidant, antimicrobial and phytochemical analysis of Cichorium intybus seeds extract and various organic fractions. Iranian Journal of Pharmaceutical Research 11, 1145-51.
Mohsenipour, Z. and Hassanshahian, M. (2015). The effects of Allium sativum extracts on biofilm formation and activities of six pathogenic bacteria. Jundishapur Journal of Microbiology 8, e18971. https://doi:10.5812/jjm.18971v2
Park, S.J., Lee, D., Kim, D., Lee, M., In, G., Han, S.T., Kim, S.W., Lee, M.H., Kim, O.K. and Lee, J. (2020). The non-saponin fraction of Korean Red Ginseng (KGC05P0) decreases glucose uptake and transport in vitro and modulates glucose production via down-regulation of the PI3K/AKT pathway in vivo. Journal of Ginseng Research 44, 362-72. https://doi:10.1016/j.jgr.2019.12.004
Powell, W.A., Catranis, C.M. and Maynard, C.A. (2000). Design of self‐processing antimicrobial peptides for plant protection. Letters in Applied Microbiology 31, 163-68. https://doi: 10.1046/j.1365-2672.2000.00782.x
Quan, H.Y. and Do Yeon Kim and S.H.C. (2013). Korean red ginseng extract alleviates advanced glycation end product-mediated renal injury. Journal of Ginseng Research 37,187. https://doi: 10.5142/jgr.2013.37.187.
Rahman, A.U., Choudhary, M.I. and Thomsen, W.J. (2001). Bioassay techniques for drug development. CRC Press.
Ramjan, A., Hossain, M., Runa, J.F., Md, H. and Mahmodul, I. (2014). Evaluation of thrombolytic potential of three medicinal plants available in Bangladesh, as a potent source of thrombolytic compounds. Avicenna Journal of Phytomedicine 4, 430-36.
Sabir, S., Anjum, A.A., Ijaz, T. and Ali, M.A. (2014). Isolation and antibiotic susceptibility of E. coli from urinary tract infections in a tertiary care hospital. Pakistan Journal of Medical Sciences 30, 389-95.
Shahrajabian, M.H., Sun, W. and Cheng, Q. (2019a). A review of ginseng species in different regions as a multipurpose herb in traditional Chinese medicine, modern herbology and pharmacological science. Journal of Medicinal Plants Research 13, 213-26. https://doi: 10.5897/JMPR2019.6731
Shahrajabian, M.H., Sun, W., Cheng, Q. (2019b). The power of natural Chinese medicine, ginger and ginseng root in an organic life. Middle-East Journal of Scientific Research 27, 64-71. https://doi: 10.5829/idosi.mejsr.2019.64.71
Shin, H.S., Yu, M., Kim, M., Choi, H.S. and Kang, D.H. (2014). Renoprotective effect of red ginseng in gentamicin-induced acute kidney injury. Laboratory Investigation 94, 1147-60. https://doi: 10.1038/labinvest.2014.101
Shamim, M. and Khan, N.I. (2019). Neuroprotective effect of Panax ginseng extract against cerebral ischemia–reperfusion-injury-induced oxidative stress in middle cerebral artery occlusion models. Facets 4, 52-68. https://doi.org/10.1139/facets-2018-0025
Siddique, N.A., Mujeeb, M., Najmi, A.K. and Akram, M. (2010). Evaluation of antioxidant activity, quantitative estimation of phenols and flavonoids in different parts of Aegle marmelos. African Journal of Plant Sciences 4, 1-5.
Souri, E.G., Amin, G., Farsan, H., Jalalizadeeh, H. and Barezi, S. (2008). Screening of thirteen medicinal plants extracts for antioxidant activity. Iranian Journal of Pharmaceutical Research 7, 149-54.
Subramanian, R., Asmawi, M.Z. and Sadikun, A. (2008). In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide. Acta Biochimica Polinica 55, 391-98.
Uluısık, D. and Keskin, E. (2016). Hepatoprotective effects of ginseng in rats fed cholesterol rich diet. Acta Scientiae Veterinariae 44, 1346.
Xiao-Ping, Y.E., Chun-Qing, S.O.N.G., Ping, Y.U.A.N. and Ren-Gang, M.A.O. (2010). α-glucosidase and α-amylase inhibitory activity of common constituents from traditional Chinese medicine used for diabetes mellitus. Chinese Journal of Natural Medicines 8, 349-52. https://doi.org/10.1016/S1875-5364(10)60041-6
Zhao, B., Lv, C. and Lu, J. (2019). Natural occurring polysaccharides from Panax ginseng CA Meyer: A review of isolation, structures, and bioactivities. International Journal of Biological Macromolecules 133, 324‐36. https://doi:10.1016/j.ijbiomac.2019.03.229.
Published
2020-08-12
How to Cite
Hussain, F., Akram, A., Hafeez, J., & Shahid, M. (2020). Biofunctional characterization of red, black and white ginseng (Panax ginseng Meyer) root extracts. Revista Mexicana De Ingeniería Química, 20(1), 173-184. https://doi.org/10.24275/rmiq/Bio1735
Section
Biotechnology