Antagonistic microorganisms efficiency to suppress damage caused by Colletotrichum gloeosporioides in papaya crop: Perspectives and challenges

Keywords: microbial antagonists, biological control, postharvest disease, papaya, C. gloeosporioides


Papaya (Carica papaya L.) is one of the most valued tropical fruits worldwide due to its nutritional content. Its production is drastically affected by Colletotrichum gloeosporioides, one of the main pathogens responsible for anthracnose disease. Several techniques as an alternative of conventional chemical treatments for disease control have been studied. Among these techniques, the use of antagonist microorganism has emerged as a promising, eco-friendly alternative for postharvest disease control. This review is focused on the inhibition of Colletotrichum gloeosporioides in papaya applying microbial antagonists. Furthermore, the main purpose of this study is to provide the results of in vivo and in vitro assays, that addressed the use of microorganisms and their activity as biocontrol agents in papaya, considering its application to diminish crop losses and suggesting possible future researches addressed to their attractive usage. We believe that a specific compilation is helpful for groups that are in research of pre- and postharvest fruits management, providing useful information to create new perspectives and/or alternative in emerging technologies.


Adams, P.B. (1990). The potential of mycoparasites for biological control of plant diseases. Annual Review of Phytopathology 28, 59-72.
Akem, C.N. (2006). Mango anthracnose disease: Present status and future research priorities. Plant Pathology Journal 5, 266-273.
Awang, Y., Ghani, M.A.A., Sijam, K. and Mohamad, R.B. (2011). Effect of calcium chloride on anthracnose disease and postharvest quality of red-flesh dragon fruit (Hylocereus polyrhizus). African Journal of Microbiology Research 5, 5250-5259.
Baños-Guevara, P.E., Zavaleta-Mejía, E., Colinas-León, M.T., Luna-Romero, I. and Gutiérrez-Alonso, J.G. (2004). Control biológico de Colletotrichum gloeosporioides [(Penz.) Penz. y Sacc.] en papaya Maradol roja (Carica papaya L.) y fisiología postcosecha de frutos infectados. Revista Mexicana de Fitopatología 22, 198-205. Available at:
Barhoom, S. and Sharon, A. (2004). cAMP regulation of “pathogenic” and “saprophytic” fungal spore germination. Fungal Genetics and Biology 41, 317-326.
Bautista-Baños, S., Sivakumar, D., Villanueva-Arce, A., Hernández-López, R. and Bello-Pérez, M. (2013). A review of the management alternatives for controlling fungi on papaya fruit during the postharvest supply chain. Crop Protection 49, 8-20.
Bautista-Rosales, P.U., Calderón-Santoyo, M., Servín-Villegas, R., Ochoa-Álvarez, N.A. and Ragazzo-Sánchez, J.A. (2013). Action mechanisms of the yeast Meyerozyma caribbica for the control of the phytopathogen Colletotrichum gloeosporioides in mangoes. Biological Control 65, 293-301.
Carballo-Sánchez, M. P., Ramírez-Ramírez, J. C., Gimeno, M., Hall, G. M., Ríos-Durán, M. G., and Shirai, K. (2016). Papaya (Carica papaya) and tuna (Thunnus albacares) by-products fermentation as biomanufacturing approach towards antioxidant protein hydrolysates. Revista Mexicana de Ingeniería Química 15(1), 91-100. Available at:,%20No.%201/Alim3/Alim3.html
Chávez-Magdaleno, M.E., Luque-Alcaraz, A.G., Gutiérrez-Martínez, P., Cortez-Rocha, M.O., Burgos-Hernández, A., Lizardi-Mendoza, J., & Plascencia-Jatomea, M. (2018). Effect of chitosan-pepper tree (Schinus molle) essential oil biocomposites on the growth kinetics, viability and membrane integrity of Colletotrichum gloeosporioides. Revista Mexicana de Ingeniería Química 17, 29-45.
Choudhary, B., Nagoure, A. and Gupta, R. (2015). Biological control of toxigenic citrus and papaya-rotting fungi by Streptomyces violascens MT7 and its extracellular metabolites. Journal of Basic Microbiology 55, 1343-1356.
Cook, R.J. and Baker, K.F. (1983). The nature and practice of biological control of plant pathogens. APS Press: St. Paul, Minnesota.
de Capdeville, G., Souza, M.T., Pereira, J.R., Paula, S., Rodrigues, A. and Gonçalves, F.A. (2007a). Selection and testing of epiphytic yeasts to control anthracnose in post-harvest of papaya fruit. Scientia Horticulturae 111, 179-185.
de Capdeville, G., Souza, M.T., Pereira, J.R., Paula, S., Rodrigues, A., Falcão, R. and Mendes, A.C.M. (2007b). Scanning electron microscopy of the interaction between Cryptococcus magnus and Colletotrichum gloeosporioides on papaya fruit. Pesquisa Agropecuária Brasileira 42, 1537-1544. Available at:
Fravel, D.R. (1988). Role of antibiosis in the biocontrol of plant diseases. Annual Review of Phytopathology 26, 75-91.
Freeman, S. and Shabi, E. (1996). Cross-infection of subtropical and temperate fruits by Colletotrichum species from various hosts. Physiological and Molecular Plant Pathology 49, 395-404.
Gamagae, S.U., Sivakumar, D., Wilson-Wijeratnam, R.S. and Wijesundera, R.L.C. (2003). Use of sodium bicarbonate and Candida oleophila to control anthracnose in papaya during storage. Crop Protection 22, 775-779.
Gamagae, S.U., Sivakumar, D. and Wijesundera, R.L.C. (2004). Evaluation of postharvest application of sodium bicarbonate-incorporated wax formulation and Candida oleophila for the control of anthracnose of papaya. Crop Protection 23, 575-579.
González-Estrada, R.R., Ascencio-Valle, F., Ragazzo-Sánchez, J.A. and Calderón-Santoyo, M. (2017a). Use of a marine yeast as a biocontrol agent of the novel pathogen Penicillium citrinum on Persian lime. Emirates Journal of Food and Agriculture 29,114-122.
González-Estrada, R.R., Carvajal-Millán, E., Ragazzo-Sánchez, J.A., Bautista-Rosales, P.U. and Calderón-Santoyo, M. (2017b). Control of blue mold decay on Persian lime: Application of covalently cross-linked arabinoxylans bioactive coatings with antagonistic yeast entrapped. LWT- Food Science and Technology 85, 187-196.
Handelsman, J. and Parke, J.L. (1989). Mechanisms in biocontrol of soilborne plant pathogens. In: Plant-microbe interactions, molecular and genetic perspectives, (T. Kosuge and E.W. Nester, eds.), Pp. 27-61. McGraw-Hill: New York.
Hernández-Montiel, L.G., Rueda-Puente, E.O., Córdoba-Matson, M.V., Holguín-Peña, J.R. and Zulueta-Rodríguez, R. (2013). Mutualistic interaction of rhizobacteria with arbuscular mycorrhizal fungi and its antagonistic effect on Fusarium oxysporum in Carica papaya seedlings. Crop Protection 47, 61-63.
Kadir, J., Rahman, M.A., Mahmud, T.M.M., Abdul Rahman, R. and Begum, M.M. (2008). Extraction of antifungal substances from Burkholderia cepacia with antibiotic activity against Colletotrichum gloeosporioides on papaya (Carica papaya). International Journal of Agriculture and Biology 10, 15-20. Available at:
Landero-Valenzuela, N., Lara-Viveros, F.M., Andrade-Hoyos, P., Aguilar-Pérez, L.A. and Aguado-Rodríguez, G.J. (2016). Alternativas para el control de Colletotrichum spp. Revista Mexicana de Ciencias Agrícolas 7, 1189-1198.
LeClair, E., Conner, R., Robinson, D. and Gillard, C.L. (2015). Transmission of anthracnose (Colletotrichum lindemuthianum) in dry bean (Phaseolus vulgaris L.) with artificial and natural inoculum in a wet and dry canopy. Canadian Journal of Plant Science 95, 913-921.
Lima, J.R., Gondim, D.M.F., Oliveira, J.T.A., Oliveira, F.S.A., Goncalves, L.R.B. and Viana, F.M.P. (2013). Use of killer yeast in the management of postharvest papaya anthracnose. Postharvest Biology and Technology 83, 58-64.
Luján-Hidalgo, M.C., Rustrian-Castellanos, I.E., Arias-Castro, C., Gutiérrez-Miceli, F.A., Rodríguez-Mendiola, M.A., Mancilla-Margalli, N.A., Abud-Archila, M. (2019). Microencapsulation of microbial consortium and volatile compounds of palm (Acrocomia aculeata) wine. Revista Mexicana de Ingeniería Química 18(3), 1245-1259.
Magallón-Andalón, C.G., Luna-Solano, G., Ragazzo-Sánchez, J.A. and Calderón-Santoyo, M. (2012). Parasitism and substrate competitions effect of antagonistic yeasts for biocontrol of Colletotrichum gloeosporioides in papaya (Carica papaya L.) var Maradol. Mexican Journal of Scientific Research 1, 2-9.
Marquez-Zequera, I., Cruz-Lachica, I., Ley-Lopez, N., Carrillo-Facio, J. A., Osuna-Garcia, L. A., and Garcia-Estrada, R. S. (2018). First report of Carica papaya fruit anthracnose caused by Colletotrichum fructicola in Mexico. Plant Disease 102(12), 2649.
Osman, M.S., Sivakumar, D. and Korsten, L. (2010). Effect of biocontrol agent Bacillus amyloliquefaciens and 1-methyl cyclopropene on the control of postharvest diseases and maintenance of fruit quality. Crop Protection 30, 173-178.
Paull, R.E., Nishijima, W., Reyes, M. and Cavaletto, C. (1997). Postharvest handling and losses during marketing of papaya (Carica papaya). Postharvest Biology and Technology 11, 165-179.
Rahman, M.A., Kadir, J., Mahmud, T.M.M., Abdul Rahman, R. and Begun, M.M. (2007). Screening of antagonistic bacteria for biocontrol activities on Colletotricum gloesporioides in papaya. Asian Journal of Plant Sciences 6, 12-20.
Rahman, M.A., Mahmud, T.M.M., Kadir, J., Abdul Rahman, R. and Begun, M.M. (2009). Enhancing the efficacy of Burkholderia cepacia B23 with calcium chloride and chitosan to control anthracnose of papaya during storage. The Plant Pathology Journal 25, 361-368.
Roberts, R.G. (1994). Integrating biological control into postharvest disease management strategies. HortScience 29, 758-762.
Rojo-Báez, I., García-Estrada, R.S., Sañudo-Barajas, A.J., León-Félix, J. and Allende-Molar, R. (2016). Proceso de infección de antracnosis por Colletotrichum truncatum en papaya Maradol. Revista Brasileira de Fruticultura, [online] 39, p-379. Available at: [Accessed 8 Nov. 2018].
Salman, A., Tsror, L., Pomerantz, A., Moreh, R., Mordechai, S. and Huleihel, M. (2010). FTIR spectroscopy for detection and identification of fungal phytopathogenes. Spectroscopy 24, 261-267.
Sangeetha, C.G. and Rawal, R.D. (2008). Nutritional studies of Colletotrichum gloeosporioides (Penz.) Penz. and Sacc. The incitant of mango anthracnose. World Journal of Agricultural Sciences 4, 717-720. Available at:
Sharma, M. and Kulshrestha, S. (2015). Colletotrichum gloeosporioides: An anthracnose causing pathogen of fruits and vegetables. Biosciences Biotechnology Research Asia 12, 1233-1246.
Shi, J., Liu, A., Li, X., Feng, S. and Chen, W. (2011) Inhibitory mechanisms induced by the endophytic bacterium MGY2 in controlling anthracnose of papaya. Biological Control 56, 2-8.
Siddiqui, Y. and Ali, A. (2014). Colletotrichum gloeosporioides (Anthracnose). In: Postharvest decay, (S. Bautista-Baños, ed.) Pp. 337-371. Elsevier, México.
Sotelo-Boyás, M.E., Valverde-Aguilar, G., Plascencia-Jatomea, M., Correa-Pacheco, Z.N., Jiménez-Aparicio, A., Solorza-Feria, J., Barrera-Necha, L., Bautista-Baños, S. (2015). Characterization of chitosan nanoparticles added with essential oils. In vitro effect on Pectobaterium carotovorum. Revista Mexicana de Ingeniería Química 14, 589-599. Available at:,%20No.%203/Alim3/Alim3.html
Tapia-Tussell, R., Quijano-Ramayo, A., Cortes-Velázquez, A., Lappe, P., Larque-Saavedra, A. and Perez-Brito, D. (2008). PCR-based detection and characterization of the fungal pathogens Colletotrichum gloeosporioides and Colletotrichum capsici causing anthracnose in papaya (Carica papaya L.). Yucatán Peninsula. Molecular Biotechnology 40, 293-298.
Tapia-Tussell, R., Cortés-Velázquez, A., Valencia-Yah, T., Navarro, C., Espinosa, E., Moreno and B., Perez-Brito, D. (2016) First report of Colletotrichum magnum causing anthracnose in papaya in Mexico. Plant Disease 100, 2323.
Torres-Calzada, C., Tapia-Tussell, R., Higuera-Ciapara, I. and Pérez-Brito, D. (2013). Morphological, pathological and genetic diversity of Colletotrichum species responsible for anthracnose in papaya (Carica papaya L). European Journal of Plant Pathology 135, 67-79.

Tronsmo, A. and Dennis, C. (1977). The use of Trichoderma species to control strawberry fruit rots. Netherlands Journal of Plant Pathology 83, 449-455.
How to Cite
Silva-Jara, J., López-Cruz, R., Ragazzo-Sánchez, J., & Calderón-Santoyo, M. (2019). Antagonistic microorganisms efficiency to suppress damage caused by Colletotrichum gloeosporioides in papaya crop: Perspectives and challenges. Revista Mexicana De Ingeniería Química, 19(2), 839-849.