PRODUCTION OF FRUCTOOLIGOSACCHARIDES BY TWO NEW RECOMBINANT β-FRUCTOFURANOSIDASE ENZYMES FROM MEZCAL FERMENTING YEASTS

  • J.H. Gómez-Angulo
  • L. Amaya-Delgado
  • A. Gschaedler-Mathis
  • J. Arrizon-Gaviño
Keywords: Candida apicola, Torulaspora delbrueckii, β-fructofuranosidases, Fructooligosaccharides

Abstract

The gene Cap inv32 from Candida apicola and Tde inv32 from Torulaspora delbrueckii were identified as β-fructofuranosidase belonging to glycoside hydrolases family 32, with an opening reading frame of 1593 base pairs and 1743 base pairs, and a molecular weight of 59 kDa and 65 kDa, for Cap Inv32 and Tde Inv32 correspondingly. These enzymes contained the motif characteristics of the GH32 and showed an identity of 40 % (Cap Inv32) and 52.4 % (Tde Inv32) with an invertase of S. cerevisiae. The fructosyltransferase activity was tasted obtaining for Cap Inv32 the quantity of FOS 12.4 gL-1 and 9.03 gL-1 with 420 gL-1 and 700 gL-1, the production of FOS with Tde Inv32 was 14.61 gL-1 (420 gL-1) and 13.3 gL-1 (700 gL-1). Cap Inv32 and Tde Inv32 are β-fructofuranosidase with fructosyltransferase activity, with potential to industrial applications, focused in FOS production.

Published
2018-06-26
How to Cite
Gómez-Angulo, J., Amaya-Delgado, L., Gschaedler-Mathis, A., & Arrizon-Gaviño, J. (2018). PRODUCTION OF FRUCTOOLIGOSACCHARIDES BY TWO NEW RECOMBINANT β-FRUCTOFURANOSIDASE ENZYMES FROM MEZCAL FERMENTING YEASTS. Revista Mexicana De Ingeniería Química, 17(3), 1039-1049. Retrieved from http://rmiq.org/ojs311/index.php/rmiq/article/view/8
Section
Food Engineering