PHYSICOCHEMICAL, MORPHOLOGICAL AND STRUCTURAL CHARACTERIZATION OF THE CHITIN AND CHITOSAN OF Tenebrio molitor AND Galleria mellonella INSECTS

  • A.I. Sáenz-Mendoza
  • P.B. Zamudio-Flores
  • G.A. Palomino-Artalejo
  • J.M. Tirado-Gallegos
  • V.G. García-Cano
  • J.J. Ornelas-Paz
  • C. Ríos-Velasco
  • C.H. Acosta-Muñiz
  • A. Vargas-Torres
  • R. Salgado-Delgado
Keywords: Lepidoptera, coleoptera, α-chitin, HPLC-SEC, biopolymer materials.

Abstract

In the present study, chitin and chitosan were isolated from flour obtained from adult specimens of Tenebrio molitor and Galleria mellonella. The yield of chitin was 11.6% and 6.2% for T. molitor and G. mellonella, respectively. The degree of deacetylation of chitosan was 80.5% and 88.5% for G. mellonella and T. molitor, respectively. The content of nitrogen (≈ 6.5%), humidity (≈ 5.8%) and ashes (≈ 0.4%) in the chitin and chitosan of both insects indicated an acceptable quality. No differences were observed between the molecular weights of the chitin (≈ 889 kDa) and the chitosan (≈ 254 kDa) of both insects. Scanning electron microscopy revealed fiber structures in the chitin and chitosan from T. molitor; in contrast, the chitin and chitosan of G. mellonella were spongy. Infrared spectroscopy and X-ray diffraction showed α-type polymorphism in the chitin of both insects. The biopolymers of G. mellonella exhibited a higher crystallinity index than those of T. molitor. Due to the higher yield obtained from T. molitor, this insect could be considered as a promising alternative source for obtaining chitin and chitosan.

Published
2018-08-11
How to Cite
Sáenz-Mendoza, A., Zamudio-Flores, P., Palomino-Artalejo, G., Tirado-Gallegos, J., García-Cano, V., Ornelas-Paz, J., Ríos-Velasco, C., Acosta-Muñiz, C., Vargas-Torres, A., & Salgado-Delgado, R. (2018). PHYSICOCHEMICAL, MORPHOLOGICAL AND STRUCTURAL CHARACTERIZATION OF THE CHITIN AND CHITOSAN OF Tenebrio molitor AND Galleria mellonella INSECTS. Revista Mexicana De Ingeniería Química, 18(1), 39-56. Retrieved from http://rmiq.org/ojs311/index.php/rmiq/article/view/85
Section
Biotechnology