Detection of Salmonella enterica on silicon substrates biofunctionalized with anti-Salmonella IgG, analyzed by FTIR spectroscopy

  • F. J. Gómez-Montaño
  • A. Orduña-Díaz
  • M.C. G. Avelino-Flores
  • F. Avelino-Flores
  • F. Ramos-Collazo
  • C. Reyes-Betanzo
  • V. López-Gayou
Keywords: biosensor, silicon, FTIR, Salmonella, IgG

Abstract

Foodborne diseases are considered a health problem, Salmonella is part of the main microorganisms that generate this type of diseases, so its detection requires a short period of time, biosensors can be a good option to meet this need. In this work, three substrates (crystalline and amorphous silicon, hydrogenated amorphous silicon carbide) were biofunctionalized applying self-assembled monolayers technique, presence of characteristic functional groups of the assembly was verified by Fourier transform infrared spectroscopy (FTIR). Anti-Salmonella IgG antibodies against whole bacteria were generated. Second derivative of each FTIR detection spectrum was obtained, having modifications in the zone between 1600-1700 cm-1 given by the secondary structure of proteins, being crystalline silicon and hydrogenated amorphous silicon carbide platforms, which presented the most notorious bands. Detection was performed on flat substrates with a specific area (25 mm2), a detection time of 60 min was stablished and a concentration of 500 CFU/mL was detected. With this, the three silicon and derivatives biofunctionalized may have future applications in food microbial quality monitoring.

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Published
2020-01-12
How to Cite
Gómez-Montaño, F. J., Orduña-Díaz, A., Avelino-Flores, M. G., Avelino-Flores, F., Ramos-Collazo, F., Reyes-Betanzo, C., & López-Gayou, V. (2020). Detection of Salmonella enterica on silicon substrates biofunctionalized with anti-Salmonella IgG, analyzed by FTIR spectroscopy. Revista Mexicana De Ingeniería Química, 19(3), 1175-1185. https://doi.org/10.24275/rmiq/Bio993