Bio24352 Vol. 23 No. 3 (2024)

Vol. 23, No. 3 (2024), Bio24352 https://doi.org/10.24275/rmiq/Bio24352

Polyhydroxyalkanoates production by Bacillus thuringiensis HA1 using sugarcane molasses as carbon source

Authors

J.D. Castilla-Marroquín, N. Pacheco, J.A. Herrera-Corredor, F. Hernández-Rosas, K. Jiménez-Morales, M. J. Benítez-Salamanca, R. Hernández-Martínez

Abstract

Polyhydroxyalkanoates (PHAs) are a biodegradable alternative to conventional plastics. However, their adoption has been limited by high production costs. The use of cheap carbon sources, like by-products from the sugar cane industry, could reduce PHAs production costs. In the present work, the potential of Bacillus thuringiensis HA1 to produce PHAs using commercial sucrose, molasses, and panela as low-cost carbon sources was explored. Single factor experiments were performed to identify the most favorable conditions for PHAs production. Based on the time, temperature and initial pH results, the fixed conditions to evaluate the carbon sources were 36 hours, 33°C and pH7.5, the carbon sources were tested at 50 to 350 g/L for PHAs production. PHAs recovery ratio was higher when using molasses at 300 g/L (61.6% of PHAs/dry cell weight, 2.28 mg/mL/3.72 mg/mL respectively), followed by sucrose (61.5% of PHAs/dry cell weight, 0.4 mg/mL/0.6 mg/mL respectively) at 50 g/L and 36.4% of PHAs/dry cell weight at 300 g/L, and finally using panela 25.8% of PHAs/dry cell (1.08 mg/mL/4.40 mg/mL respectively) recovery ratio was observed. After conducting FT-IR characterization, the samples exhibited similarity to the standard DL-β-hydroxybutyric acid sodium salt

Keywords

biopolymers, polyhydroxyalkanoates, Bacillus thuringiensis, submerged fermentation, carbon sources.

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Ferrari, F., Striani, R., Fico, D., Alam, M., Greco, A., and Corcione, C. (2022). An overview on wood waste valorization as biopolymers and biocomposites: definition, classification, production, properties and applications. Polymers 14(24), 5519. https://doi.org/10.3390/polym14245519
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