Vol. 23, No. 2 (2024), Bio24204 https://doi.org/10.24275/rmiq/Bio24204

Evaluation of a vermicompost leachate as a mixotrophic culture medium for Scenedesmus acutiformis in a column photobioreactor with potential for biodiesel production

 

Authors

A.G. Zenteno-Carballo, Y.D.C. Pérez-Luna, J. Pantoja-Enríquez, R. Berrones-Hernández, S. Saldaña-Trinidad, E. G. Gómez-Vàzquez, M.A. Canseco-Pérez, Y. Sánchez-Roque

Abstract

Currently, efforts are being made to establish low-cost and easily scalable cultivation systems, through the use of alternative culture media is studied, such as leachates from the degradation of organic material with a variety of nutrients that can be assimilated by microalgae. Therefore, in this research, the lipid yield of Scenedesmus acutiformis were evaluated in a column photobioreactor of 3 liters for 30 days, using as a medium cultivate a vermicompost leachate at a total nitrogen concentration of 0.5 mgL-1, from the compost consisting residues of papaya, green grass, dry grass, almond leaves and bovine manure, with nitrogen concentrations of 0.23, 0.55, 0.93, 0.67 and 1.13%, respectively. Three concentrations (15, 25 and 50%) were prepared with two lighting levels (46.25 and 64.75 μmol photons m−2 s−1) and photoperiod 12/12, were evaluated.  The best treatment was leachate at 50%, produced 28.7% lipids. The profile of fatty acids showed linolenic acid as the most abundant (27.01%), followed by linoleic acid, palmitic acid, stearic acid, oleic acid, palmitoleic acid at concentrations of 17.05, 16.46, 14.70, 12.75 and 3.69%, respectively. The results obtained show the potential of vermicompost leachates standardized and characterized as a culture medium for the production of lipids.

Keywords

mixotrophic, leached, photobioreactor, photoperiod, biomass.

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