Revista Mexicana de Ingeniería Química, Vol. 22, No. 3 (2023), Alim2319

Screening of main factors in microencapsulation of two Bifidobacterium strains by spray drying

A.J. Borrás-Enríquez, J.L. Gonzalez-Escobar, R.E. Delgado-Portales, M.R. Pérez-Barba, M. Moscosa-Santillán



Microencapsulation of Bifidobacterium infantis and Bifidobacterium lactis by spray drying has been studied. This work aimed to screen operational factors affecting the survival percentage, the probiotic powder yield, and activity water. Parameters such as air inlet temperature (AIT), air inlet flow rate (AIF), core material type (CM), core material concentration (CMC), and Lactic Acid Bacteria (LAB) were screened using a Taguchi design of experiments. The results indicated that the most influential parameters on survival percentage were LAB and AIF (p<0.05). On the other hand, all powders exhibited a high concentration of microorganisms (>8 log CFU/gds) and low activity water (aw<0.3), establishing essential features for their use as probiotic powder and stability for their storage. Moreover, the results demonstrated that encapsulation by spray drying using skim milk reconstituted and β-cyclodextrin-Gum arabic effectively increased the survival of Bifidobacterium strains in the gastro-intestinal tract. Therefore, this microencapsulation process could allow better colonization into the intestine for probiotic effects assurance on consumer health.

Keywords: taguchi screening design, spray drying, microencapsulation, Bifidobacterium, cell viability.



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