Revista Mexicana de Ingeniería Química, Vol. 22, No. 3 (2023), Bio23112

Survival of alginate-microencapsulated Lacticaseibacillus paracasei under gastrointestinal conditions in models in vitro and in vivo

C.A. Gómez-Aldapa, J. Castro-Rosas, F.A. Guzmán-Ortiz, O.A. Acevedo-Sandoval, E. Rangel-Vargas, R.N. Falfán-Cortés



The aim of this work was to evaluate the probiotic potential of the strain Lacticaseibacillus paracasei, both free and microencapsulated by ionic gelation, using in vitro and in vivo models. The strain was microencapsulated with different alginate concentrations (1, 2, 3, and 4 %). After encapsulation, the following were evaluated: survival (%), morphology, particle size (µm), zeta potential, resistance to pH and bile salts, and adhesion of free and encapsulated bacteria in CD-1 mice. The highest resistance to simulated pH 2 and bile salt conditions was obtained with 2 % alginate. The microsphere size ranged between 47.43 and 72.56 µm, with zeta potential from –11.7 to –22.00 (Mv) and oval morphology. The quantification results in mice intestines showed that free and encapsulated bacteriaadhered at concentrations of 3 and 6 Log CFU/g (p ≤ 0.05), respectively. L. paracasei is a potential probiotic in the food and pharmaceutical industries.

Keywords: alginate, probiotics, CD-1 mice, microencapsulation, zeta potential.



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