IA25552 Vol. 24 No. 2 (2025)

Vol. 24, No. 2 (2025), IA25552 https://doi.org/10.24275/rmiq/IA25552

Association of pyocyanin and LED red-light irradiation (700 nm) on the biodegradation of waste lube oil

Authors

H. Borchardt, R. Leite, A.A.P. Mendes, I.P.G. Amaral, U. Vasconcelos

Abstract

Billions of liters of Waste Lube Oil (WLO) are produced every year and affect the environment. Pseudomonas aeruginosa is a versatile bacterium that can be used in processes to remove pollutants from the environment. Its bioactive pyocyanin (PYO) is a blue pigment with multiple cellular functions including a role in the assimilation of hydrocarbons. This study assessed the association of pyocyanin and LED light irradiation (700±10 nm) in the reduction of WLO by P. aeruginosa TGC04. Microcosms containing 45 mL of mineral medium combined with 5% WLO (v/v) were added to 5 mL of P. aeruginosa TGC04, suspended in 0,9% saline solution, adjusting the turbidity to Optical Density (OD) 0.4 at 600 nm, and different concentrations of PYO (0.1; 1.0 and 10.0 µg/mL). The microcosms were incubated at 29±1°C for 30 days under continuous LED light irradiation (700±10 nm). Control was carried out in the dark. WLO reduction was calculated by gravimetric method. Abiotic losses (≈ 10%) were determined with uninoculated microcosms. Light stimulated the inoculum, reducing the WLO from 17 to 55%. The process was PYO concentration-dependent and significantly influenced by the inoculum. There was an important correlation between PYO and WLO degradation by P. aeruginosa TGC04. In contrast, the interactions between light irradiation and PYO, as well as light irradiation alone, were insignificant possibly due to photoinactivation. These results highlight the biotechnological applicability of P. aeruginosa metabolites for ex situ bioremediation.

Keywords

Bioremediation, Oil hydrocarbons, Pseudomonas aeruginosa, Bioactive pigments.

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