Vol. 25, No. 1 (2026), Bio26680 https://doi.org/10.24275/rmiq/Bio26680

Soybean-based media for the cultivation of Bacillus subtilis 105 as a bio-fungicide against Sclerotinia sclerotiorum

 

Authors

L.G. Sarmiento-López, M. López-Meyer, I.E. Maldonado-Mendoza, G. Sepúlveda-Jiménez, M. Rodríguez-Monroy

Abstract

Bacillus subtilis 105is a bacterium with biocontrol potential against Sclerotinia sclerotiorum, a plant pathogen. To achieve the mass production of B. subtilis, this study proposes the use of agro-industrial byproducts to prepare an alternative medium to the microbiological Luria Bertani (LB). Three different media were prepared: Soybean flour, yeast extract, and NaCl (SYM); Pea flour, yeast extract, and NaCl (PYM); Solulys, yeast extract, and NaCl SolYM). SYM was selected for use in a shake flask as a suitable alternative to the microbiological LB medium because it results in the highest cell density. The selected SYM was evaluated for its ability to grow B. subtilis in a stirred tank bioreactor, and the effects 400 and 700 rpm in a bioreactor were assessed. The growth of B. subtilis grown at 400 rpm was similar in SYM media and microbiological LB, but oxygen limitation was observed. At 700 rpm, oxygen limitation was improved. Notably, the highest antagonistic activity against S. sclerotiorum was observed with B. subtilis cultures grown in SYM at 400 rpm. In conclusion, SYM is an innovation to use of microbiological LB medium for the production of B. subtilis in bioreactors while preserving its antagonistic activity against S. sclerotiorum.

Keywords

Stirred tank bioreactor, Bacillus subtillis, Sclerotinia sclerotiorum, Low-cost medium, Soybean.

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