Vol. 25, No. 1 (2026), IA26665 https://doi.org/10.24275/rmiq/IA26665

Synergistic effect of high hydrostatic pressure, hydrogen peroxide, and a Trametes hirsuta BM-2 enzymatic extract on polyphenol release from Sargassum spp.

 

Authors

M. E. González-Muñoz, K. D. Chikani-Cabrera, E. I. España-Gamboa, P. Machado Bueno-Fernandes, R. Tapia-Tussell, L. Alzate-Gaviria

Abstract

Massive influxes of Sargassum spp. in the Mexican Caribbean, exacerbated by pollution and climate change, pose severe environmental challenges but also represent a promising resource for biorefineries. This study assessed the effect of high hydrostatic pressure (HHP) combined with chemical and enzymatic pretreatments on polyphenol release from Sargassum fluitans and Sargassum natans. Biomass was subjected to HHP (0.101–200 MPa) either alone or with hydrogen peroxide (H₂O₂), a crude enzymatic extract from Trametes hirsuta BM-2, or their sequential application. HHP alone produced no significant effect, but in combination with pretreatments it markedly enhanced polyphenol extraction. Maximum concentrations reached 8.10 and 7.54 mg GAE eq g⁻¹ TS for peroxide and enzymatic treatments at 150 MPa, while the sequential process achieved 16.18 mg GAE eq g⁻¹ TS. Comparable yields using individual treatments at 50 and 150 MPa indicate potential energy savings for scale-up. These results demonstrate that coupling pretreatments with HHP significantly improves phenolic recovery, offering a sustainable strategy to valorize invasive sargassum biomass and reduce its coastal impact.

Keywords

Sargassum spp., high hydrostatic pressure, polyphenols, hydrogen peroxide, laccase, delignification.

References
  • Albuquerque, E. D., Torres, F. A. G., Fernandes, A. A. R., & Fernandes, P. M. B. (2016). Combined effects of high hydrostatic pressure and specific fungal cellulase improve coconut husk hydrolysis. Process Biochemistry 51(11), 1767-1775. https://doi.org/10.1016/j.procbio.2016.07.010
  • Cañas, A. I., & Camarero, S. (2010). Laccases and their natural mediators: Biotechnological tools for sustainable eco-friendly processes. Biotechnology Advances 28(6), 694-705. https://doi.org/10.1016/j.biotechadv.2010.05.002
  • Chávez, V., Uribe-Martínez, A., Cuevas, E., Rodríguez-Martínez, R. E., van Tussenbroek, B. I., Francisco, V., Estévez, M., Celis, L. B., Monroy-Velázquez, L. V., Leal-Bautista, R., Álvarez-Filip, L., García-Sánchez, M., Masia, L., & Silva, R. (2020). Massive Influx of Pelagic Sargassum spp. on the Coasts of the Mexican Caribbean 2014–2020: Challenges and Opportunities. Water 12(10), 2908. https://doi.org/10.3390/w12102908
  • Chikani-Cabrera, K. D., Fernandes, P. M. B., Tapia-Tussell, R., Parra-Ortiz, D. L., Hernández-Zárate, G., Valdez-Ojeda, R., & Alzate-Gaviria, L. (2022). Improvement in Methane Production from Pelagic Sargassum Using Combined Pretreatments. Life 12(8), 1214. https://doi.org/10.3390/life12081214
  • Escobar, F.A., Salcedo-Pérez, E., Pérez-Martínez, M.E., Serrato-Díaz, A., (2024). Biochar-packed biofilter for the treatment of gases produced by accumulated Sargassum waste. Revista Mexicana de Ingeniería Química 23, 637-653. https://doi.org/10.24275/rmiq/IA676
  • Gisbert, M., Franco, D., Sineiro, J., & Moreira, R. (2023). Antioxidant and Antidiabetic Properties of Phlorotannins from Ascophyllum nodosum Seaweed Extracts. Molecules 28(13), Article 13. https://doi.org/10.3390/molecules28134937
  • Gray, L. A., Bisonó León, A. G., Rojas, F. E., Veroneau, S. S., & Slocum, A. H. (2021). Caribbean-Wide, Negative Emissions Solution to Sargassum spp. Low-Cost Collection Device and Sustainable Disposal Method. Phycology 1(1), 49-75. https://doi.org/10.3390/phycology1010004
  • Hofrichter, M., Ullrich, R., Pecyna, M. J., Liers, C., & Lundell, T. (2010). New and classic families of secreted fungal heme peroxidases. Applied Microbiology and Biotechnology 87(3), 871-897. https://doi.org/10.1007/s00253-010-2633-0
  • Jard, G., Marfaing, H., Carrère, H., Delgenes, J. P., Steyer, J. P., & Dumas, C. (2013). French Brittany macroalgae screening: Composition and methane potential for potential alternative sources of energy and products. Bioresource Technology 144, 492-498. https://doi.org/10.1016/j.biortech.2013.06.114
  • Jiang, H., Kong, L., Tang, H., Wang, Z., Liu, C., Zhang, J., Chen, Y., Shen, J., & Zhou, Y. (2024). Study on the preparation and enzyme inhibitory activity of polyphenols from Sargassum pallidum. PLOS One 19(1), e0297434. https://doi.org/10.1371/journal.pone.0297434
  • Lee, H.-H., Kim, J.-S., Jeong, J.-H., Lee, S.-Y., & Kim, C.-S. (2022). Comparative Analysis of Biological Activities and Phenolic Content between Fresh and Steamed Sargassum fusiforme in Different Extraction Solvents. Applied Sciences 12(23), Article 23. https://doi.org/10.3390/app122312161
  • Loan, D.T.T., García-Gutiérrez, J., Aguilar-González, M.A., Hernández-Cortez, C., Luna-González, A., López-Martínez, L.X., (2024). Impact of ultrasound-assisted enzymatic extraction of polyphenols from purple rice bran. Revista Mexicana de Ingeniería Química 23, 279-292. https://doi.org/10.24275/rmiq/Bio2761
  • Ma, M., & Mu, T. (2016). Modification of deoiled cumin dietary fiber with laccase and cellulase under high hydrostatic pressure. Carbohydrate Polymers 136, 87-94. https://doi.org/10.1016/j.carbpol.2015.09.030
  • Moiseenko, K. V., Glazunova, O. A., Savinova, O. S., & Fedorova, T. V. (2023). Exoproteomic Study and Transcriptional Responses of Laccase and Ligninolytic Peroxidase Genes of White-Rot Fungus Trametes hirsuta LE-BIN 072 Grown in the Presence of Monolignol-Related Phenolic Compounds. International Journal of Molecular Sciences 24(17), 13115. https://doi.org/10.3390/ijms241713115
  • Navarro-Baez, J. E., Martínez, L. M., Welti-Chanes, J., Buitimea-Cantúa, G. V., & Escobedo-Avellaneda, Z. (2022). High Hydrostatic Pressure to Increase the Biosynthesis and Extraction of Phenolic Compounds in Food: A Review. Molecules 27(5), 1502. https://doi.org/10.3390/molecules27051502
  • Prasedya, E. S., Frediansyah, A., Martyasari, N. W. R., Ilhami, B. K., Abidin, A. S., Padmi, H., Fahrurrozi, Juanssilfero, A. B., Widyastuti, S., & Sunarwidhi, A. L. (2021). Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract. Scientific Reports 11(1), 17876. https://doi.org/10.1038/s41598-021-95769-y
  • Puspita, M., Déniel, M., Widowati, I., Radjasa, O. K., Douzenel, P., Marty, C., Vandanjon, L., Bedoux, G., & Bourgougnon, N. (2017). Total phenolic content and biological activities of enzymatic extracts from Sargassum muticum (Yendo) Fensholt. Journal of Applied Phycology, 29(5) 2521-2537. https://doi.org/10.1007/s10811-017-1086-6
  • Resiere, D., Mehdaoui, H., Florentin, J., Gueye, P., Lebrun, T., Blateau, A., Viguier, J., Valentino, R., Brouste, Y., Kallel, H., Mégarbane, B., Cabié, A., Banydeen, R., & Neviere, R. (2021). Sargassum seaweed health menace in the Caribbean: Clinical characteristics of a population exposed to hydrogen sulfide during the 2018 massive stranding. Clinical Toxicology 59, 215–223. https://doi.org/10.1080/15563650.2020.1789162
  • Rodrigues, D., Freitas, A. C., Queirós, R., Rocha-Santos, T. A. P., Saraiva, J. A., Gomes, A. M. P., & Duarte, A. C. (2017). Bioactive Polysaccharides Extracts from Sargassum muticum by High Hydrostatic Pressure. Journal of Food Processing and Preservation 41(1), e12977. https://doi.org/10.1111/jfpp.12977
  • Rodríguez-Martínez, R. E., Torres-Conde, E. G., Rosellón-Druker, J., Cabanillas-Terán, N., & Jáuregui-Haza, U. (2025). The Great Atlantic Sargassum Belt: Impacts on the Central and Western Caribbean–A review. Harmful Algae 144, 102838.https://doi.org/10.1016/j.hal.2025.102838
  • Santana-Martí, L. A., Rey-Villiers, N., Pi-Puig, T., Rodríguez-Martínez, R. E., & Sánchez, A. (2026). Impact of pelagic Sargassum on calcareous biocomponents and Mg-calcite content in Northern Mexican Caribbean sediments. CATENA 262, 109624.https://doi.org/10.1016/j.catena.2025.109624
  • Shunmugiah Mahendran, Subbiah Sankaralingam, Senthurpandian Muthuramalinga Sethu, Durairaj Kathiresan, Mahalingam Muthumani, Loganathan Kousalya, Selvam Palpperumal, & Balasundaram Harinathan. (2024). Evaluation of antioxidant and cytotoxicity activities of polyphenol extracted from brown seaweed Sargassum tenerrimum biomass. Biomass Conversion and Biorefinery 14(2), 2063-2069. https://doi.org/10.1007/s13399-022-02301-x
  • Tapia-Tussell, R., Avila-Arias, J., Domínguez Maldonado, J., Valero, D., Olguin-Maciel, E., Pérez-Brito, D., & Alzate-Gaviria, L. (2018). Biological Pretreatment of Mexican Caribbean Macroalgae Consortiums Using Bm-2 Strain (Trametes hirsuta) and Its Enzymatic Broth to Improve Biomethane Potential. Energies 11(3), 494. https://doi.org/10.3390/en11030494
  • Teong, S. P., Li, X., & Zhang, Y. (2019). Hydrogen peroxide as an oxidant in biomass-to-chemical processes of industrial interest. Green Chemistry 21(21), 5753-5780. https://doi.org/10.1039/c9gc02445j
  • Vijayan, R., Chitra, L., Penislusshiyan, S., & Palvannan, T. (2018). Exploring bioactive fraction of Sargassum wightii: In vitro elucidation of angiotensin-I-converting enzyme inhibition and antioxidant potential. International Journal of Food Properties 21(1), 674-684. https://doi.org/10.1080/10942912.2018.1454465
  • Wan, C., Jiang, H., Tang, M.-T., Zhou, S., & Zhou, T. (2022). Purification, physico-chemical properties and antioxidant activity of polysaccharides from Sargassum fusiforme by hydrogen peroxide/ascorbic acid-assisted extraction. International Journal of Biological Macromolecules 223, 490-499. https://doi.org/10.1016/j.ijbiomac.2022.11.030