Vol. 23, No. 3 (2024), IA24251 https://doi.org/10.24275/rmiq/IA24251

Effect of exogenous phenazine addition on crude heavy oil degradation by Pseudomonas aeruginosa TGC04

 

Authors

A. A. Galvão-Viana, H. Borchardt, J. V. Dantas, D. S. Bernardes-Dias, I. P. Gurgel-Amaral, U. Vasconcelos

Abstract

Previous studies have shown that phenazines contribute to hydrocarbons (HC) transformation by Pseudomonas aeruginosa. The rodis capable of degrading HC shortly after exposure to oil; and this study aimed to assess the effect of phenazine methosulfate (PMS), on the removal of HC by bioaugmentation with pyocyanin-producing P. aeruginosa TGC04. Microcosms were prepared containing fine sand and oil-contaminated beach sand (1:4). The pre-inoculum was prepared with pasteurized sand enriched with 10 µmol/L of PMS; supplemented with barley malt bagasse and incubated at 29±1°C for 10 days. Afterwards, portions of the inoculum were added to the microcosms (1:10; 1:100 and 1:1000). The total petroleum hydrocarbons (TPH) were reduced by up to ≈49%, while the 16 USEPA priority polycyclic aromatic hydrocarbons (PAHs) were reduced by ≈37 and 56%. P. aeruginosa TGC04 preferentially degraded 4-6 ring PAHs (80-89%). The 2-3 ring PAHs were removed by up to ≈37%. In the presence of PMS, there was a significant reduction in HC; the highest rates of degradation, however, were observed without PMS (1:100). As a contribution, this study expands the knowledge that the hydrocarbonoclastic activity of P. aeruginosa is not increased by addition of exogenous phenazines but favors the removal of 4-6 ring PAHs.

Keywords

Bioremediation, Allochthonous bioaugmentation, Encompassed inoculum, Phenazine Methosulphate, Polycyclic Aromatic Hydrocarbons.

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