Bio25551 Vol. 24 No. 3 (2025)

Vol. 24, No. 3 (2025), Bio25551 https://doi.org/10.24275/rmiq/Bio25551

Biodegradation potential of thermophilic cellulolytic bacteria isolated from urban organic waste composting

Authors

A. Avila-Andrade, R. Beristain-Cardoso, D. García-Mondragón, S. Alcaraz-Ibarra, J.F. Aguirre-Garrido, G. González-Blanco

Abstract

The composting process of urban organic waste presents a microbial diversity depending on the phases of the process, which makes it a source of isolation of thermostable and hydrolytic bacteria of biotechnological importance due to their ability to degrade complex compounds such as cellulose, hemicellulose, and lignin. The objective of this study was to isolate and characterize thermophilic cellulolytic bacteria, as well as to determine their hydrolytic and degradation potential. Microbial isolation was performed from compost samples in the thermophile phase (47°C ± 2.25). Identification was performed by biochemical tests and massive amplicon sequencing. Hydrolytic capacity was determined by hydrolysis halos and kinetic activity in 0.1% CMC medium at 50°C. Bacillus sp. and Bacillus licheniformis strains were isolated from a thermophilic-phase composting system. These strains exhibited specific growth rates of 0.1051 h-1 and 0.0794 h-1, doubling times of 6.5 h and 8.8 h, and hydrolysis halo diameters of 1.08 cm and 0.8 cm, respectively. The identification of bacteria of the Bacillus genus from the composting process highlights its importance as a source of thermophilic bacteria with hydrolytic capacity for biotechnological applications.

Keywords

Isolation, composting, thermophilic, bacilli, cellulose, residue.

References
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